Richard B. Gillis
A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology
Gillis, Richard B.; Rowe, Arthur J.; Adams, Gary G.; Harding, Stephen E.
Arthur J. Rowe
Gary G. Adams
STEPHEN HARDING firstname.lastname@example.org
Professor of Applied Biochemistry
This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.
Gillis, R. B., Rowe, A. J., Adams, G. G., & Harding, S. E. (2015). A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology. Biotechnology and Genetic Engineering Reviews, 30(2), doi:10.1080/02648725.2014.994870
|Journal Article Type||Article|
|Acceptance Date||Dec 3, 2014|
|Online Publication Date||Feb 16, 2015|
|Publication Date||Mar 5, 2015|
|Deposit Date||May 11, 2017|
|Journal||Biotechnology and Genetic Engineering Reviews|
|Publisher||Taylor & Francis|
|Peer Reviewed||Peer Reviewed|
|Keywords||analytical ultracentrifugation, SEC-MALS, viscometry, light scattering, hydrodynamics, biopolymer, polydisperse|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf|
This file is under embargo due to copyright reasons.
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