Skip to main content

Research Repository

Advanced Search

A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology

Gillis, Richard B.; Rowe, Arthur J.; Adams, Gary G.; Harding, Stephen E.

Authors

Arthur J. Rowe

Gary G. Adams



Abstract

This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.

Journal Article Type Article
Publication Date Mar 5, 2015
Journal Biotechnology and Genetic Engineering Reviews
Print ISSN 0264-8725
Electronic ISSN 0264-8725
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
Volume 30
Issue 2
APA6 Citation Gillis, R. B., Rowe, A. J., Adams, G. G., & Harding, S. E. (2015). A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology. Biotechnology and Genetic Engineering Reviews, 30(2), doi:10.1080/02648725.2014.994870
DOI https://doi.org/10.1080/02648725.2014.994870
Keywords analytical ultracentrifugation, SEC-MALS, viscometry, light scattering, hydrodynamics, biopolymer, polydisperse
Publisher URL http://www.tandfonline.com/doi/full/10.1080/02648725.2014.994870
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
;