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A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology

Gillis, Richard B.; Rowe, Arthur J.; Adams, Gary G.; Harding, Stephen E.

Authors

Richard B. Gillis

Arthur J. Rowe

Gary G. Adams



Abstract

This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.

Citation

Gillis, R. B., Rowe, A. J., Adams, G. G., & Harding, S. E. (2015). A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology. Biotechnology and Genetic Engineering Reviews, 30(2), doi:10.1080/02648725.2014.994870

Journal Article Type Article
Acceptance Date Dec 3, 2014
Online Publication Date Feb 16, 2015
Publication Date Mar 5, 2015
Deposit Date May 11, 2017
Journal Biotechnology and Genetic Engineering Reviews
Print ISSN 0264-8725
Electronic ISSN 0264-8725
Publisher Taylor & Francis
Peer Reviewed Peer Reviewed
Volume 30
Issue 2
DOI https://doi.org/10.1080/02648725.2014.994870
Keywords analytical ultracentrifugation, SEC-MALS, viscometry, light scattering, hydrodynamics, biopolymer, polydisperse
Public URL http://eprints.nottingham.ac.uk/id/eprint/42765
Publisher URL http://www.tandfonline.com/doi/full/10.1080/02648725.2014.994870
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf