A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology

Gillis, Richard B.; Rowe, Arthur J.; Adams, Gary G.; Harding, Stephen E.

Abstract

This short review considers the range of modern techniques for the hydrodynamic characterisation of macromolecules – particularly large glycosylated systems used in the food, biopharma and healthcare industries. The range or polydispersity of molecular weights and conformations presents special challenges compared to proteins. The review is aimed, without going into any great theoretical or methodological depth, to help the Industrial Biotechnologist choose the appropriate methodology or combination of methodologies for providing the detail he/she needs for particular applications.

Citation

Gillis, R. B., Rowe, A. J., Adams, G. G., & Harding, S. E. (2015). A review of modern approaches to the hydrodynamic characterisation of polydisperse macromolecular systems in biotechnology. Biotechnology and Genetic Engineering Reviews, 30(2), https://doi.org/10.1080/02648725.2014.994870

• Journal Article Type: Article
• Acceptance Date: Dec 3, 2014
• Online Publication Date: Feb 16, 2015
• Publication Date: Mar 5, 2015
• Deposit Date: May 11, 2017
• Journal: Biotechnology and Genetic Engineering Reviews
• Print ISSN: 0264-8725
• Electronic ISSN: 0264-8725
• Publisher: Taylor & Francis Open
• Peer Reviewed: Peer Reviewed
• Volume: 30
• Issue: 2
• DOI: https://doi.org/10.1080/02648725.2014.994870
• Keywords: analytical ultracentrifugation, SEC-MALS, viscometry, light scattering, hydrodynamics, biopolymer, polydisperse
• Public URL: https://nottingham-repository.worktribe.com/output/748051
• Publisher URL: http://www.tandfonline.com/doi/full/10.1080/02648725.2014.994870