Drosophila heart cell movement to the midline occurs through both cell autonomous migration and dorsal closure

Haack, Timm; Schneider, Matthias; Schwendele, Bernd; Renault, Andrew D.

Abstract

The Drosophila heart is a linear organ formed by the movement of bilaterally specified progenitor cells to the midline and adherence of contralateral heart cells. This movement occurs through the attachment of heart cells to the overlying ectoderm which is undergoing dorsal closure. Therefore heart cells are thought to move to the midline passively. Through live imaging experiments and analysis of mutants that affect the speed of dorsal closure we show that heart cells in Drosophila are autonomously migratory and part of their movement to the midline is independent of the ectoderm. This means that heart formation in flies is more similar to that in vertebrates than previously thought. We also show that defects in dorsal closure can result in failure of the amnioserosa to properly degenerate, which can physically hinder joining of contralateral heart cells leading to a broken heart phenotype.

Citation

Haack, T., Schneider, M., Schwendele, B., & Renault, A. D. (2014). Drosophila heart cell movement to the midline occurs through both cell autonomous migration and dorsal closure. Developmental Biology, 396(2), https://doi.org/10.1016/j.ydbio.2014.08.033

• Journal Article Type: Article
• Acceptance Date: Aug 30, 2014
• Online Publication Date: Sep 16, 2014
• Publication Date: Dec 15, 2014
• Deposit Date: Jun 15, 2016
• Publicly Available Date: Jun 15, 2016
• Journal: Developmental Biology
• Print ISSN: 0012-1606
• Electronic ISSN: 0012-1606
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 396
• Issue: 2
• DOI: https://doi.org/10.1016/j.ydbio.2014.08.033
• Keywords: Dorsal vessel; Heart; Drosophila; wunen; Amnioserosa; Dorsal closure; Organogenesis
• Public URL: https://nottingham-repository.worktribe.com/output/740854
• Publisher URL: http://dx.doi.org/10.1016/j.ydbio.2014.08.033
• Contract Date: Jun 15, 2016