Study of NAP adsorption and assembly on the surface of HOPG

Korolkov, Vladimir V.; Allen, Stephanie; Roberts, Clive J.; Gozes, Illana; Tendler, Saul J.B.

Abstract

NAP is an octapeptide that has demonstrated a neuroprotective/therapeutic efficacy at very low concentrations in preclinical studies and in a number of clinical trials. Yet little is known about its structural organization at low concentrations. Here, we have employed atomic force microscopy to investigate NAP peptide assembly on graphite in aqueous media at nanomolar concentration. High spatial resolution scans of NAP assemblies reveal their fine structure with clearly resolved single NAP units. This observation leads us to conclude that NAP molecules do not form complex self-assembled structures at nanomolar concentration when adsorbed on graphite surface.

Citation

Korolkov, V. V., Allen, S., Roberts, C. J., Gozes, I., & Tendler, S. J. (2014). Study of NAP adsorption and assembly on the surface of HOPG. Peptides, 62, 55-58. https://doi.org/10.1016/j.peptides.2014.09.017

• Journal Article Type: Article
• Acceptance Date: Sep 17, 2014
• Online Publication Date: Sep 30, 2019
• Publication Date: 2014-12
• Deposit Date: Oct 24, 2014
• Publicly Available Date: Dec 31, 2014
• Journal: Peptides
• Print ISSN: 0196-9781
• Electronic ISSN: 0196-9781
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 62
• Pages: 55-58
• DOI: https://doi.org/10.1016/j.peptides.2014.09.017
• Keywords: NAP; Atomic Force Microscopy; davunetide; adsorption; peptides
• Public URL: https://nottingham-repository.worktribe.com/output/735047
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0196978114002861
• Additional Information: This article is maintained by: Elsevier; Article Title: Study of NAP adsorption and assembly on the surface of HOPG; Journal Title: Peptides; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.peptides.2014.09.017; Content Type: article; Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.