Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse

Kydd, Julia H.; Case, Ruth; Minke, Julius; Audonnet, Jean-Christophe; Wagner, Bettina; Antczak, Douglas F.

Abstract

Cytotoxic T-lymphocytes (CTLs) are associated with protective immunity against disease caused by equid herpesvirus type 1 (EHV-1). However, the EHV-1 target proteins for CTLs are poorly defined. This limits the development of vaccine candidates designed to stimulate strong CTL immunity. Here, classical CTL assays using lymphocytes from horses of three defined MHC class I types that experienced natural infection with EHV-1 and a modified vaccinia virus construct containing an EHV-1 gene encoding the immediate-early (IE) protein are reported. Horses homozygous for the equine leukocyte antigen (ELA)-A2 haplotype, but not the ELA-A5 haplotype, produced MHC-restricted CTL responses against the IE protein. Previously, horses homozygous for the ELA-A3 haplotype also mounted CTL responses against the IE protein. Both haplotypes are common in major horse breeds, including the Thoroughbred. Thus, the IE protein is an attractive candidate molecule for future studies of T-cell immunity to EHV-1 in the horse.

Citation

Kydd, J. H., Case, R., Minke, J., Audonnet, J., Wagner, B., & Antczak, D. F. (in press). Immediate early protein of equid herpesvirus type 1 as a target for cytotoxic T-lymphocytes in the thoroughbred horse. Journal of General Virology, 95, https://doi.org/10.1099/vir.0.065888-0

• Journal Article Type: Article
• Acceptance Date: May 13, 2014
• Online Publication Date: Aug 1, 2014
• Deposit Date: Jul 21, 2016
• Publicly Available Date: Jul 21, 2016
• Journal: Journal of General Virology
• Print ISSN: 0022-1317
• Electronic ISSN: 1465-2099
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 95
• DOI: https://doi.org/10.1099/vir.0.065888-0
• Keywords: Equine, Herpesvirus, Thoroughbred, Cytotoxic T lymphocytes
• Public URL: https://nottingham-repository.worktribe.com/output/731429
• Publisher URL: http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.065888-0
• Additional Information: This is the author's version of a work that was accepted for publication in Journal of General Virology. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of General Virology Vol. 95, pp. 1783-1789 and is available at http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.065888-0