LEAH BAND leah.band@nottingham.ac.uk
Associate Professor
Systems Analysis of Auxin Transport in the Arabidopsis Root Apex
Band, Leah R.; Wells, Darren M.; Fozard, John A.; Ghetiu, Teodor; French, Andrew P.; Pound, Michael P.; Wilson, Michael H.; Yu, Lei; Li, Wenda; Hijazi, Hussein I.; Oh, Jaesung; Pearce, Simon P.; Perez-Amador, Miguel A.; Yun, Jeonga; Kramer, Eric; Alonso, Jose M.; Godin, Christophe; Vernoux, Teva; Hodgman, T. Charlie; Pridmore, Tony P.; Swarup, Ranjan; King, John R.; Bennett, Malcolm J.
Authors
DARREN WELLS DARREN.WELLS@NOTTINGHAM.AC.UK
Principal Research Fellow
John A. Fozard
Teodor Ghetiu
ANDREW FRENCH andrew.p.french@nottingham.ac.uk
Associate Professor
MICHAEL POUND Michael.Pound@nottingham.ac.uk
Assistant Professor
MICHAEL WILSON MICHAEL.WILSON@NOTTINGHAM.AC.UK
Future Food Beacon:Technologist in Bioinformatics/Computational Biology
Lei Yu
Wenda Li
Hussein I. Hijazi
Jaesung Oh
Simon P. Pearce
Miguel A. Perez-Amador
Jeonga Yun
Eric Kramer
Jose M. Alonso
Christophe Godin
Teva Vernoux teva.vernoux@ens-lyon.fr
T. Charlie Hodgman
TONY PRIDMORE tony.pridmore@nottingham.ac.uk
Professor of Computer Science
RANJAN SWARUP RANJAN.SWARUP@NOTTINGHAM.AC.UK
Associate Professor
JOHN KING john.king@nottingham.ac.uk
Professor of Theoretical Mechanics
MALCOLM BENNETT malcolm.bennett@nottingham.ac.uk
Professor of Plant Science
Abstract
Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the cellular scale is essential to understanding how these processes are controlled. In this study, we developed an auxin transport model based on actual root cell geometries and carrier subcellular localizations. We tested model predictions using the DII-VENUS auxin sensor in conjunction with state-of-the-art segmentation tools. Our study revealed that auxin efflux carriers alone cannot create the pattern of auxin distribution at the root tip and that AUX1/LAX influx carriers are also required. We observed that AUX1 in lateral root cap (LRC) and elongating epidermal cells greatly enhance auxin’s shootward flux, with this flux being predominantly through the LRC, entering the epidermal cells only as they enter the elongation zone. We conclude that the nonpolar AUX1/LAX influx carriers control which tissues have high auxin levels, whereas the polar PIN carriers control the direction of auxin transport within these tissues.
Citation
Band, L. R., Wells, D. M., Fozard, J. A., Ghetiu, T., French, A. P., Pound, M. P., …Bennett, M. J. (2014). Systems Analysis of Auxin Transport in the Arabidopsis Root Apex. Plant Cell, 26(3), 862-875. https://doi.org/10.1105/tpc.113.119495
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 14, 2014 |
Online Publication Date | Mar 14, 2014 |
Publication Date | Mar 14, 2014 |
Deposit Date | Oct 18, 2016 |
Publicly Available Date | Oct 18, 2016 |
Journal | Plant Cell |
Print ISSN | 1040-4651 |
Electronic ISSN | 1532-298X |
Publisher | American Society of Plant Biologists |
Peer Reviewed | Peer Reviewed |
Volume | 26 |
Issue | 3 |
Pages | 862-875 |
DOI | https://doi.org/10.1105/tpc.113.119495 |
Public URL | http://eprints.nottingham.ac.uk/id/eprint/37628 |
Publisher URL | http://www.plantcell.org/content/26/3/862 |
Copyright Statement | Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf |
Files
Apex root 862.full.pdf
(2.7 Mb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf
You might also like
Auxin fluxes through plasmodesmata modify root-tip auxin distribution
(2020)
Journal Article
Cellular patterning of Arabidopsis roots under low phosphate conditions
(2018)
Journal Article