Systems Analysis of Auxin Transport in the Arabidopsis Root Apex

Band, Leah R.; Wells, Darren M.; Fozard, John A.; Ghetiu, Teodor; French, Andrew P.; Pound, Michael P.; Wilson, Michael H.; Yu, Lei; Li, Wenda; Hijazi, Hussein I.; Oh, Jaesung; Pearce, Simon P.; Perez-Amador, Miguel A.; Yun, Jeonga; Kramer, Eric; Alonso, Jose M.; Godin, Christophe; Vernoux, Teva; Hodgman, T. Charlie; Pridmore, Tony P.; Swarup, Ranjan; King, John R.; Bennett, Malcolm J.

Abstract

Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the cellular scale is essential to understanding how these processes are controlled. In this study, we developed an auxin transport model based on actual root cell geometries and carrier subcellular localizations. We tested model predictions using the DII-VENUS auxin sensor in conjunction with state-of-the-art segmentation tools. Our study revealed that auxin efflux carriers alone cannot create the pattern of auxin distribution at the root tip and that AUX1/LAX influx carriers are also required. We observed that AUX1 in lateral root cap (LRC) and elongating epidermal cells greatly enhance auxin’s shootward flux, with this flux being predominantly through the LRC, entering the epidermal cells only as they enter the elongation zone. We conclude that the nonpolar AUX1/LAX influx carriers control which tissues have high auxin levels, whereas the polar PIN carriers control the direction of auxin transport within these tissues.

Citation

Band, L. R., Wells, D. M., Fozard, J. A., Ghetiu, T., French, A. P., Pound, M. P., Wilson, M. H., Yu, L., Li, W., Hijazi, H. I., Oh, J., Pearce, S. P., Perez-Amador, M. A., Yun, J., Kramer, E., Alonso, J. M., Godin, C., Vernoux, T., Hodgman, T. C., Pridmore, T. P., …Bennett, M. J. (2014). Systems Analysis of Auxin Transport in the Arabidopsis Root Apex. Plant Cell, 26(3), 862-875. https://doi.org/10.1105/tpc.113.119495

• Journal Article Type: Article
• Acceptance Date: Feb 14, 2014
• Online Publication Date: Mar 14, 2014
• Publication Date: Mar 14, 2014
• Deposit Date: Oct 18, 2016
• Publicly Available Date: Oct 18, 2016
• Journal: Plant Cell
• Print ISSN: 1040-4651
• Electronic ISSN: 1532-298X
• Publisher: American Society of Plant Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 26
• Issue: 3
• Pages: 862-875
• DOI: https://doi.org/10.1105/tpc.113.119495
• Public URL: https://nottingham-repository.worktribe.com/output/724981
• Publisher URL: http://www.plantcell.org/content/26/3/862
• Contract Date: Oct 18, 2016