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Systems Analysis of Auxin Transport in the Arabidopsis Root Apex

Band, Leah R.; Wells, Darren M.; Fozard, John A.; Ghetiu, Teodor; French, Andrew P.; Pound, Michael P.; Wilson, Michael H.; Yu, Lei; Li, Wenda; Hijazi, Hussein I.; Oh, Jaesung; Pearce, Simon P.; Perez-Amador, Miguel A.; Yun, Jeonga; Kramer, Eric; Alonso, Jose M.; Godin, Christophe; Vernoux, Teva; Hodgman, T. Charlie; Pridmore, Tony P.; Swarup, Ranjan; King, John R.; Bennett, Malcolm J.

Authors

LEAH BAND leah.band@nottingham.ac.uk
Associate Professor

DARREN WELLS DARREN.WELLS@NOTTINGHAM.AC.UK
Principal Research Fellow

John A. Fozard

Teodor Ghetiu

MICHAEL WILSON MICHAEL.WILSON@NOTTINGHAM.AC.UK
Future Food Beacon:Technologist in Bioinformatics/Computational Biology

Lei Yu

Wenda Li

Hussein I. Hijazi

Jaesung Oh

Simon P. Pearce

Miguel A. Perez-Amador

Jeonga Yun

Eric Kramer

Jose M. Alonso

Christophe Godin

Teva Vernoux teva.vernoux@ens-lyon.fr

T. Charlie Hodgman

TONY PRIDMORE tony.pridmore@nottingham.ac.uk
Professor of Computer Science

JOHN KING john.king@nottingham.ac.uk
Professor of Theoretical Mechanics



Abstract

Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the cellular scale is essential to understanding how these processes are controlled. In this study, we developed an auxin transport model based on actual root cell geometries and carrier subcellular localizations. We tested model predictions using the DII-VENUS auxin sensor in conjunction with state-of-the-art segmentation tools. Our study revealed that auxin efflux carriers alone cannot create the pattern of auxin distribution at the root tip and that AUX1/LAX influx carriers are also required. We observed that AUX1 in lateral root cap (LRC) and elongating epidermal cells greatly enhance auxin’s shootward flux, with this flux being predominantly through the LRC, entering the epidermal cells only as they enter the elongation zone. We conclude that the nonpolar AUX1/LAX influx carriers control which tissues have high auxin levels, whereas the polar PIN carriers control the direction of auxin transport within these tissues.

Journal Article Type Article
Publication Date Jan 1, 2014
Journal Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher American Society of Plant Biologists
Peer Reviewed Peer Reviewed
Volume 26
Issue 3
Pages 862-875
APA6 Citation Band, L. R., Wells, D. M., Fozard, J. A., Ghetiu, T., French, A. P., Pound, M. P., …Bennett, M. J. (2014). Systems Analysis of Auxin Transport in the Arabidopsis Root Apex. Plant Cell, 26(3), 862-875. https://doi.org/10.1105/tpc.113.119495
DOI https://doi.org/10.1105/tpc.113.119495
Publisher URL http://www.plantcell.org/content/26/3/862
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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