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Nitric oxide sensing in plants is mediated by proteolytic control of Group VII ERF transcription factors

Gibbs, Daniel J.; Md Isa, Nurulhikma; Movahedi, Mahsa; Lozano-Juste, Jorge; Mendiondo, Guillermina M.; Berckhan, Sophie; Marín-de la Rosa, Nora; Vicente Conde, Jorge; Sousa Correia, Cristina; Pearce, Simon P.; Bassel, George W.; Hamali, Bulut; Talloji, Prabhavathi; Tomé, Daniel F.A.; Coego, Alberto; Beynon, Jim; Alabadí, David; Bachmair, Andreas; León, José; Gray, Julie E.; Theodoulou, Frederica L.; Holdsworth, Michael J.

Authors

Daniel J. Gibbs

Nurulhikma Md Isa

Mahsa Movahedi

Jorge Lozano-Juste

Guillermina M. Mendiondo

Sophie Berckhan

Nora Marín-de la Rosa

Jorge Vicente Conde

Cristina Sousa Correia

Simon P. Pearce

George W. Bassel

Bulut Hamali

Prabhavathi Talloji

Daniel F.A. Tomé

Alberto Coego

Jim Beynon

David Alabadí

Andreas Bachmair

José León

Julie E. Gray

Frederica L. Theodoulou

Michael J. Holdsworth

Abstract

Nitric oxide (NO) is an important signaling compound in prokaryotes and eukaryotes. In plants, NO regulates critical developmental transitions and stress responses. Here, we identify a mechanism for NO sensing that coordinates responses throughout development based on targeted degradation of plant-specific transcriptional regulators, the group VII ethylene response factors (ERFs). We show that the N-end rule pathway of targeted proteolysis targets these proteins for destruction in the presence of NO, and we establish them as critical regulators of diverse NO-regulated processes, including seed germination, stomatal closure, and hypocotyl elongation. Furthermore, we define the molecular mechanism for NO control of germination and crosstalk with abscisic acid (ABA) signaling through ERF-regulated expression of ABSCISIC ACID INSENSITIVE5 (ABI5). Our work demonstrates how NO sensing is integrated across multiple physiological processes by direct modulation of transcription factor stability and identifies group VII ERFs as central hubs for the perception of gaseous signals in plants.

Journal Article Type Article
Publication Date Feb 6, 2014
Journal Molecular Cell
Print ISSN 1097-2765
Electronic ISSN 1097-4164
Publisher Elsevier (Cell Press)
Peer Reviewed Peer Reviewed
Volume 53
Issue 3
Institution Citation Gibbs, D. J., Md Isa, N., Movahedi, M., Lozano-Juste, J., Mendiondo, G. M., Berckhan, S., …Holdsworth, M. J. (2014). Nitric oxide sensing in plants is mediated by proteolytic control of Group VII ERF transcription factors. Molecular Cell, 53(3), doi:10.1016/j.molcel.2013.12.020
DOI https://doi.org/10.1016/j.molcel.2013.12.020
Publisher URL http://www.sciencedirect.com/science/article/pii/S1097276513009301
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0




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