Nitric oxide sensing in plants is mediated by proteolytic control of Group VII ERF transcription factors

Gibbs, Daniel J.; Md Isa, Nurulhikma; Movahedi, Mahsa; Lozano-Juste, Jorge; Mendiondo, Guillermina M.; Berckhan, Sophie; Mar�n-de la Rosa, Nora; Vicente Conde, Jorge; Sousa Correia, Cristina; Pearce, Simon P.; Bassel, George W.; Hamali, Bulut; Talloji, Prabhavathi; Tom�, Daniel F.A.; Coego, Alberto; Beynon, Jim; Alabad�, David; Bachmair, Andreas; Le�n, Jos�; Gray, Julie E.; Theodoulou, Frederica L.; Holdsworth, Michael J.

Abstract

Nitric oxide (NO) is an important signaling compound in prokaryotes and eukaryotes. In plants, NO regulates critical developmental transitions and stress responses. Here, we identify a mechanism for NO sensing that coordinates responses throughout development based on targeted degradation of plant-specific transcriptional regulators, the group VII ethylene response factors (ERFs). We show that the N-end rule pathway of targeted proteolysis targets these proteins for destruction in the presence of NO, and we establish them as critical regulators of diverse NO-regulated processes, including seed germination, stomatal closure, and hypocotyl elongation. Furthermore, we define the molecular mechanism for NO control of germination and crosstalk with abscisic acid (ABA) signaling through ERF-regulated expression of ABSCISIC ACID INSENSITIVE5 (ABI5). Our work demonstrates how NO sensing is integrated across multiple physiological processes by direct modulation of transcription factor stability and identifies group VII ERFs as central hubs for the perception of gaseous signals in plants.

Citation

Gibbs, D. J., Md Isa, N., Movahedi, M., Lozano-Juste, J., Mendiondo, G. M., Berckhan, S., Marín-de la Rosa, N., Vicente Conde, J., Sousa Correia, C., Pearce, S. P., Bassel, G. W., Hamali, B., Talloji, P., Tomé, D. F., Coego, A., Beynon, J., Alabadí, D., Bachmair, A., León, J., Gray, J. E., …Holdsworth, M. J. (2014). Nitric oxide sensing in plants is mediated by proteolytic control of Group VII ERF transcription factors. Molecular Cell, 53(3), https://doi.org/10.1016/j.molcel.2013.12.020

• Journal Article Type: Article
• Acceptance Date: Dec 13, 2013
• Online Publication Date: Jan 23, 2014
• Publication Date: Feb 6, 2014
• Deposit Date: Jan 24, 2017
• Publicly Available Date: Jan 24, 2017
• Journal: Molecular Cell
• Print ISSN: 1097-2765
• Electronic ISSN: 1097-4164
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 53
• Issue: 3
• DOI: https://doi.org/10.1016/j.molcel.2013.12.020
• Public URL: https://nottingham-repository.worktribe.com/output/723449
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S1097276513009301
• Contract Date: Jan 24, 2017