ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana

Zubrycka, Agata; Dambire, Charlene; Dalle Carbonare, Laura; Sharma, Gunjan; Boeckx, Tinne; Swarup, Kamal; Sturrock, Craig J.; Atkinson, Brian S.; Swarup, Ranjan; Corbineau, Françoise; Oldham, Neil J.; Holdsworth, Michael J.

Abstract

Oxygen is a key signalling component of plant biology, and whilst an oxygen-sensing mechanism was previously described in Arabidopsis thaliana, key features of the associated PLANT CYSTEINE OXIDASE (PCO) N-degron pathway and Group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factor substrates remain untested or unknown. We demonstrate that ERFVIIs show non-autonomous activation of root hypoxia tolerance and are essential for root development and survival under oxygen limiting conditions in soil. We determine the combined effects of ERFVIIs in controlling gene expression and define genetic and environmental components required for proteasome-dependent oxygen-regulated stability of ERFVIIs through the PCO N-degron pathway. Using a plant extract, unexpected amino-terminal cysteine sulphonic acid oxidation level of ERFVIIs was observed, suggesting a requirement for additional enzymatic activity within the pathway. Our results provide a holistic understanding of the properties, functions and readouts of this oxygen-sensing mechanism defined through its role in modulating ERFVII stability.

Citation

Zubrycka, A., Dambire, C., Dalle Carbonare, L., Sharma, G., Boeckx, T., Swarup, K., Sturrock, C. J., Atkinson, B. S., Swarup, R., Corbineau, F., Oldham, N. J., & Holdsworth, M. J. (2023). ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana. Nature Communications, 14, Article 4665. https://doi.org/10.1038/s41467-023-40366-y

• Journal Article Type: Article
• Acceptance Date: Jul 25, 2023
• Online Publication Date: Aug 3, 2023
• Publication Date: Aug 3, 2023
• Deposit Date: Aug 7, 2023
• Publicly Available Date: Aug 9, 2023
• Journal: Nature communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Article Number: 4665
• DOI: https://doi.org/10.1038/s41467-023-40366-y
• Keywords: General Physics and Astronomy; General Biochemistry, Genetics and Molecular Biology; General Chemistry; Multidisciplinary
• Public URL: https://nottingham-repository.worktribe.com/output/23864847
• Publisher URL: https://www.nature.com/articles/s41467-023-40366-y