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A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis in a contaminated in vivo bone defect model

McLaren, Jane S.; White, L.J.; Cox, H.C.; Ashraf, Waheed; Rahman, C.V.; Blunn, G.W.; Goodship, A.E.; Quirk, R.A.; Shakesheff, Kevin M.; Bayston, Roger; Scammell, Brigitte E.

Authors

Jane S. McLaren

L.J. White

H.C. Cox

Waheed Ashraf waheed.ashraf@nottingham.ac.uk

C.V. Rahman

G.W. Blunn

A.E. Goodship

R.A. Quirk

Kevin M. Shakesheff

Roger Bayston roger.bayston@nottingham.ac.uk

Brigitte E. Scammell

Abstract

Open fractures are at risk of serious infection and, if infected, require several surgical interventions and courses of systemic antibiotics. We investigated a new injectable formulation that simultaneously hardens in vivo to form a porous scaffold for bone repair and delivers antibiotics at high concentrations to the local site of infection. Duration of antimicrobial activity against Staphylococcus aureus was determined using the serial plate transfer test. Ultimate compressive strength and porosity of the material was measured with and without antibiotics. The material was evaluated in vivo in an ovine medial femoral condyle defect model contaminated with S. aureus. Sheep were sacrificed at either 2 or 13 weeks and the defect and surrounding bone assessed using micro-computed tomography and histology. Antimicrobial activity in vitro persisted for 19-21 days. Sheep with antibiotic-free material and bacteria became infected, while those with antibiotic-containing material and bacteria did not. Similarly, new bone growth was seen in uninoculated animals with plain polymer, and in those with antibiotic polymer with bacteria, but not in sheep with plain polymer and bacteria. The antibiotic-impregnated scaffolds were effective in preventing S. aureus infections whilst supporting bone growth and repair. If translated into clinical practice, this approach might reduce the need for systemic antibiotics.

Journal Article Type Article
Publication Date Jun 9, 2014
Journal European Cells and Materials
Electronic ISSN 1473-2262
Publisher AO Research Institute Davos
Peer Reviewed Peer Reviewed
Volume 27
Institution Citation McLaren, J. S., White, L., Cox, H., Ashraf, W., Rahman, C., Blunn, G., …Scammell, B. E. (2014). A biodegradable antibiotic-impregnated scaffold to prevent osteomyelitis in a contaminated in vivo bone defect model. eCells and Materials Journal, 27, doi:10.22203/eCM.v027a24
DOI https://doi.org/10.22203/eCM.v027a24
Keywords PLGA; biodegradable scaffold; infection; in vivo; bone ingrowth
Publisher URL http://www.ecmjournal.org/papers/vol027/pdf/v027a24.pdf
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf




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