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Peptide hydrogels — a tissue engineering strategy for the prevention of oesophageal strictures

Kumar, Deepak; Workman, Victoria; O'Brien, Marie Claire; McLaren, Jane S.; White, Lisa J.; Ragunath, Krish; Saiani, Alberto; Gough, Julie; Rose, Felicity R.A.J.

Authors

Deepak Kumar

Victoria Workman

Marie Claire O'Brien

JANE MCLAREN jane.mclaren@nottingham.ac.uk
Nottingham Senior Tissue Bank Manager

Krish Ragunath

Alberto Saiani

Julie Gough

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FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
Professor of Biomaterials and Tissue Engineering



Abstract

Endoscopic treatment of Barrett’s oesophagus often leads to further damage of healthy tissue causing fibrotic tissue formation termed as strictures. This study shows that synthetic, self-assembling peptide hydrogels (PeptiGelDesign) support the activity and function of primary oesophageal cells, leading to epithelialisation and stratification during in vitro 3D co-culture. Following buffering in culture media, oesophageal stromal fibroblasts (rOSFs) were incorporated into a library of peptide hydrogels, whereas oesophageal epithelial cells (mOECs) were seeded on the surface. Optimal hydrogels (PGD-AlphaProC and PGD-CGD2) supported mOEC viability (>95 %), typical cell morphology (cobblestone-like), a migration rate of 17.4 μm/hr and a migration distance of 364 μm, at 48 hours. Positive expression of typical epithelial markers (ZO-1 and cytokeratins) was witnessed detected using immunocytochemistry at day 3 in culture. Furthermore, optimal hydrogels were identified which supported rOSF viability (> 95%) with homogenous distribution when incorporated into the hydrogels and also promoted the secretion of collagen type I detected using ELISA, at day 7. 3D co-culture model using optimal hydrogels for both cell types supported a stratified epithelial layer (expressing involucrin and AE1/AE3 markers). Findings from this study could lead to the use of peptide hydrogels as a minimally invasive endoscopic therapy to manage oesophageal strictures.

Citation

Kumar, D., Workman, V., O'Brien, M. C., McLaren, J. S., White, L. J., Ragunath, K., …Rose, F. R. (2017). Peptide hydrogels — a tissue engineering strategy for the prevention of oesophageal strictures. Advanced Functional Materials, 27(38), Article 1702424. https://doi.org/10.1002/adfm.201702424

Journal Article Type Article
Acceptance Date Jul 11, 2017
Online Publication Date Aug 21, 2017
Publication Date Oct 12, 2017
Deposit Date Sep 8, 2017
Publicly Available Date Mar 29, 2024
Journal Advanced Functional Materials
Print ISSN 1616-301X
Electronic ISSN 1616-3028
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 27
Issue 38
Article Number 1702424
DOI https://doi.org/10.1002/adfm.201702424
Keywords Barrett's oesophagus; co-culture model; stiffness; synthetic peptide hydrogels
Public URL https://nottingham-repository.worktribe.com/output/887606
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/adfm.201702424/full
Additional Information This is the peer reviewed version of the following article: D. Kumar, V. L. Workman, M. O'Brien, J. McLaren, L. White, K. Ragunath, F. Rose, A. Saiani, J. E. Gough, Adv. Funct. Mater. 2017, 1702424, which has been published in final form at http://dx.doi.org/10.1002/adfm.201702424. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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