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Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite

Emara, Mohamed; Royer, Pierre-Joseph; Mahdavi, Jafar; Shakib, Farouk; Ghaemmaghami, Amir M.

Authors

Mohamed Emara

Pierre-Joseph Royer

Jafar Mahdavi

Farouk Shakib

Amir M. Ghaemmaghami



Abstract

Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differentiation have remained elusive. Using retagging, we identified DCSIGN as a novel receptor involved in the initial recognition and uptake of the major house dust mite and dog allergens Der p 1 and Can f 1, respectively. To confirm this, we used gene silencing
to specifically inhibit DC-SIGN expression by DCs followed by allergen uptake studies. Binding and uptake of Der p 1 and Can f 1 allergens was assessed by ELISA and flow cytometry. Intriguingly, our data showed that silencing DC-SIGN on DCs promotes a Th2 phenotype in DC/T cell co-cultures. These findings should lead to better understanding of the molecular basis of allergen-induced Th2 cell polarization and in doing so paves the way for the rational design of novel intervention strategies by targeting allergen receptors on innate immune cells or their carbohydrate counterstructures on allergens.

Journal Article Type Article
Publication Date Feb 17, 2012
Journal Journal of Biological Chemistry
Print ISSN 0021-9258
Electronic ISSN 0021-9258
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 287
Issue 8
Institution Citation Emara, M., Royer, P., Mahdavi, J., Shakib, F., & Ghaemmaghami, A. M. (2012). Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. Journal of Biological Chemistry, 287(8), doi:10.1074/jbc.M111.312520
DOI https://doi.org/10.1074/jbc.M111.312520
Keywords Immunology
Glycobiology, Extracellular Matrices
Publisher URL http://www.jbc.org/content/287/8/5756
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information This research was originally published in Journal of Biological Chemistry.
Emara, M., Royer, P-J., Mahdavi, J., Ghaemmaghami, A.M.
Retagging identifies dendritic cell-specific Intercellular
Adhesion Molecule-3 (ICAM3)-grabbing non-integrin
(DC-SIGN) protein as a novel receptor for a major allergen
from house dust mite. Journal of Biological Chemistry, 2012: 5756-5763. © the American Society for Biochemistry and Molecular Biology.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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