A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity

Brown, William R.A.; Litti, Gianni; Rosa, Carlos; James, Steve; Roberts, Ian; Robert, Vincent; Jolly, Neil; Tang, Wen; Baumann, Peter; Green, Carter; Schlegel, Kristina; Young, Jonathan; Hirchaud, Fabienne; Leek, Spencer; Thomas, Geraint; Blomberg, Anders; Warringer, Jonas

Abstract

The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell
biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent
natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces
pombe varies significantly in size but shows only limited variation in proliferation in different environments
compared with Saccharomyces cerevisiae. Nucleotide diversity, p, at a near neutral site, the central core of
the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed
karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis.
One translocation, found in 6 different isolates, including the type strain, has a geographically widespread
distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other
translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of
telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.

Citation

Brown, W. R., Litti, G., Rosa, C., James, S., Roberts, I., Robert, V., …Warringer, J. (2011). A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity. G3, 1(7), https://doi.org/10.1534/g3.111.001123

• Journal Article Type: Article
• Publication Date: Dec 1, 2011
• Deposit Date: Apr 22, 2014
• Publicly Available Date: Apr 22, 2014
• Journal: G3
• Electronic ISSN: 2160-1836
• Publisher: Genetics Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 1
• Issue: 7
• DOI: https://doi.org/10.1534/g3.111.001123
• Public URL: https://nottingham-repository.worktribe.com/output/708528
• Publisher URL: http://www.g3journal.org/content/1/7/615
• Additional Information: Published source must be acknowledged