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A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity

Brown, William R.A.; Litti, Gianni; Rosa, Carlos; James, Steve; Roberts, Ian; Robert, Vincent; Jolly, Neil; Tang, Wen; Baumann, Peter; Green, Carter; Schlegel, Kristina; Young, Jonathan; Hirchaud, Fabienne; Leek, Spencer; Thomas, Geraint; Blomberg, Anders; Warringer, Jonas

Authors

William R.A. Brown

Gianni Litti

Carlos Rosa

Steve James

Ian Roberts

Vincent Robert

Neil Jolly

Wen Tang

Peter Baumann

Carter Green

Kristina Schlegel

Jonathan Young

Fabienne Hirchaud

Spencer Leek

Geraint Thomas

Anders Blomberg

Jonas Warringer

Abstract

The fission yeast Schizosaccharomyces pombe has been widely used to study eukaryotic cell
biology, but almost all of this work has used derivatives of a single strain. We have studied 81 independent
natural isolates and 3 designated laboratory strains of Schizosaccharomyces pombe. Schizosaccharomyces
pombe varies significantly in size but shows only limited variation in proliferation in different environments
compared with Saccharomyces cerevisiae. Nucleotide diversity, p, at a near neutral site, the central core of
the centromere of chromosome II is approximately 0.7%. Approximately 20% of the isolates showed
karyotypic rearrangements as detected by pulsed field gel electrophoresis and filter hybridization analysis.
One translocation, found in 6 different isolates, including the type strain, has a geographically widespread
distribution and a unique haplotype and may be a marker of an incipient speciation event. All of the other
translocations are unique. Exploitation of this karyotypic diversity may cast new light on both the biology of
telomeres and centromeres and on isolating mechanisms in single-celled eukaryotes.

Journal Article Type Article
Publication Date Dec 1, 2011
Journal G3
Electronic ISSN 2160-1836
Publisher Genetics Society of America
Peer Reviewed Peer Reviewed
Volume 1
Issue 7
Institution Citation Brown, W. R., Litti, G., Rosa, C., James, S., Roberts, I., Robert, V., …Warringer, J. (2011). A geographically diverse collection of schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity. G3, 1(7), doi:10.1534/g3.111.001123
DOI https://doi.org/10.1534/g3.111.001123
Publisher URL http://www.g3journal.org/content/1/7/615
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information Published source must be acknowledged

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0



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