Total loss of MHC class I is an independent indicator of good prognosis in breast cancer

Madjd, Zahra; Spendlove, Ian; Pinder, Sarah E.; Ellis, Ian O.; Durrant, Lindy G.

Abstract

Tumours can be recognised by CTL and NK cells. CTL recognition depends on expression of MHC Class I loaded with peptides from tumour antigens. In contrast, loss of MHC Class I results in NK activation. In our study a large set of samples from patients with primary operable invasive breast cancer was evaluated for the expression of MHC Class I heavy and light by immunohistochemical staining of 439 breast carcinomas in a tissue microarray. Forty-seven percent (206 of 439) of breast carcinomas were considered negative for HLA Class I heavy chain (HC10), whereas lack of anti-β2m-antibody staining was observed in 39% (167 of 424) of tumours, with only 3% of the β2m-negative tumours expressing detectable HLA Class I heavy chain. Correlation with patient outcome showed direct relationship between patient survival and HLA-negative phenotype (log rank = 0.004). A positive relationship was found between the intensity of expression of MHC Class I light and heavy chains expression and histological grade of invasive tumour (p < 0.001) and Nottingham Prognostic Index (p < 0.001). To investigate whether HLA Class I heavy and light chains expression had independent prognostic significance, Cox multivariate regression analysis, including the parameters of tumour size, lymph node stage, grade and intensity of HC10 and anti-β2m staining, was carried out. In our analysis, lymph node stage (p < 0.001), tumour grade (p = 0.005) and intensity of MHC Class I light and heavy chains expression were shown to be independent prognostic factors predictive of overall survival (p-values HC10 = 0.047 and β2m = 0.018).

Citation

Madjd, Z., Spendlove, I., Pinder, S. E., Ellis, I. O., & Durrant, L. G. (2005). Total loss of MHC class I is an independent indicator of good prognosis in breast cancer. International Journal of Cancer, 117(2), https://doi.org/10.1002/ijc.21163

• Journal Article Type: Article
• Publication Date: Nov 1, 2005
• Deposit Date: May 9, 2014
• Publicly Available Date: May 9, 2014
• Journal: International Journal of Cancer
• Print ISSN: 0020-7136
• Electronic ISSN: 1097-0215
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 117
• Issue: 2
• DOI: https://doi.org/10.1002/ijc.21163
• Keywords: Breast tissue microarray, HC10, Prognostic factor, MHC Class I, Immunohistochemistry
• Public URL: https://nottingham-repository.worktribe.com/output/703470
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/ijc.21163/abstract;jsessionid=0F2D2BA7158A645CEAA0FEC990F1DD0E.f02t04