Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development

Yang, Bao Jun; Minne, Max; Brunoni, Federica; Plačková, Lenka; Petřík, Ivan; Sun, Yanbiao; Nolf, Jonah; Smet, Wouter; Verstaen, Kevin; Wendrich, Jos R.; Eekhout, Thomas; Hoyerová, Klára; Van Isterdael, Gert; Haustraete, Jurgen; Bishopp, Anthony; Farcot, Etienne; Novák, Ondřej; Saeys, Yvan; De Rybel, Bert

Abstract

During plant development, a precise balance of cytokinin is crucial for correct growth and patterning, but it remains unclear how this is achieved across different cell types and in the context of a growing organ. Here we show that in the root apical meristem, the TMO5/LHW complex increases active cytokinin levels via two cooperatively acting enzymes. By profiling the transcriptomic changes of increased cytokinin at single-cell level, we further show that this effect is counteracted by a tissue-specific increase in CYTOKININ OXIDASE 3 expression via direct activation of the mobile transcription factor SHORTROOT. In summary, we show that within the root meristem, xylem cells act as a local organizer of vascular development by non-autonomously regulating cytokinin levels in neighbouring procambium cells via sequential induction and repression modules.

Citation

Yang, B. J., Minne, M., Brunoni, F., Plačková, L., Petřík, I., Sun, Y., Nolf, J., Smet, W., Verstaen, K., Wendrich, J. R., Eekhout, T., Hoyerová, K., Van Isterdael, G., Haustraete, J., Bishopp, A., Farcot, E., Novák, O., Saeys, Y., & De Rybel, B. (2021). Non-cell autonomous and spatiotemporal signalling from a tissue organizer orchestrates root vascular development. Nature Plants, 7(11), 1485-1494. https://doi.org/10.1038/s41477-021-01017-6

• Journal Article Type: Article
• Acceptance Date: Oct 6, 2021
• Online Publication Date: Nov 15, 2021
• Publication Date: Nov 1, 2021
• Deposit Date: Nov 24, 2021
• Publicly Available Date: May 2, 2022
• Journal: Nature Plants
• Print ISSN: 2055-026X
• Electronic ISSN: 2055-0278
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 11
• Pages: 1485-1494
• DOI: https://doi.org/10.1038/s41477-021-01017-6
• Keywords: Plant Science
• Public URL: https://nottingham-repository.worktribe.com/output/6787026
• Publisher URL: https://www.nature.com/articles/s41477-021-01017-6