Myokine Responses to Exercise in a Rat Model of Low/High Adaptive Potential

Farrash, Wesam F.; Phillips, Bethan E.; Britton, Steven L.; Qi, Nathan; Koch, Lauren G.; Wilkinson, Daniel J.; Smith, Ken; Atherton, Philip J.

Abstract

Introduction: Assuming myokines underlie some of the health benefits of exercise, we hypothesised that ‘high responder trainer’ (HRT) rats would exhibit distinct myokine profiles to ‘low responder trainers’ (LRT), reflecting distinct health and adaptive traits.

Methods: Blood was collected from LRT and HRT (N=8) rats at baseline (BL), immediately (0h), 1h, and 3h after running; repeated after 3-wks training. Myokines were analysed by ELISA (i.e. BDNF/Fractalkine/SPARC/Irisin/FGF21/Musclin/IL-6).

Results: At baseline, Musclin (LRT: 84 ± 24 vs HRT: 26 ± 3 pg/ml, P=0.05) and FGF21 (LRT: 133 ± 34 vs HRT: 63.5 ± 13 pg/ml, P=0.08) were higher in LRT than HRT. Training increased Musclin in HRT (26 ± 3 to 54 ± 9 pg/ml, P less than 0.05) and decreased FGF21 in LRT (133 ± 34 to 60 ± 28 pg/ml, P less than 0.05). Training increased SPARC (LRT: 0.8 ± 0.1 to 2.1 ± 0.6 ng/ml, P less than 0.05; HRT: 0.7 ± 0.06 to 1.8 ± 0.3 ng/ml, P=0.06) and Irisin (LRT 0.62 ± 0.1 to 2.6 ± 0.4 ng/ml, P less than 0.01; HRT 0.53 ± 0.1 to 2.8 ± 0.7 ng/ml, P less than 0.01) while decreasing BDNF (LRT: 2747 ± 293 to 1081 ± 330 pg/ml, P less than 0.01; HRT: 1976 ± 328 to 797 ± 160 pg/ml, P less than 0.05). Acute exercise response of Musclin (AUC) was higher in LRT vs HRT (306 ± 74 vs. 88 ± 12 pg/ml×3h-1, P less than 0.01) and elevated in HRT after training (221 ± 31 pg/ml×3h-1, P less than 0.01). Training elevated SPARC (LRT: 2.4 ± 0.1 to 7.7 ± 1.3 ng/ml×3h-1, P less than 0.05; HRT: 2.5 ± 0.13 to 11.2 ± 2.2 ng/ml×3h-1, P less than 0.001) and Irisin (LRT: 1.34 ± 0.3 to 9.6 ± 1.7 ng/ml×3h-1, P less than 0.001; HRT: 1.5 ± 0.5 to 12.1 ± 1.9 ng/ml×3h-1, P less than 0.0001).

Conclusion: Exercise training alters how myokines are secreted in response to acute exercise. Myokine responses were not robustly linked to adaptive potential in aerobic capacity, making them an unlikely regulator of adaptive traits.

• Journal Article Type: Article
• Acceptance Date: May 17, 2021
• Online Publication Date: Jun 9, 2021
• Publication Date: Jun 9, 2021
• Deposit Date: Jun 4, 2021
• Publicly Available Date: Jun 9, 2021
• Journal: Frontiers in Endocrinology
• Electronic ISSN: 1664-2392
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Article Number: 6458891
• Pages: 1-10
• DOI: https://doi.org/10.3389/fendo.2021.645881
• Keywords: Endocrinology, Diabetes and Metabolism
• Public URL: https://nottingham-repository.worktribe.com/output/5624696
• Publisher URL: https://www.frontiersin.org/articles/10.3389/fendo.2021.645881/full