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Myokine Responses to Exercise in a Rat Model of Low/High Adaptive Potential

Farrash, Wesam F.; Phillips, Bethan E.; Britton, Steven L.; Qi, Nathan; Koch, Lauren G.; Wilkinson, Daniel J.; Smith, Ken; Atherton, Philip J.

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Authors

Wesam F. Farrash

BETH PHILLIPS beth.phillips@nottingham.ac.uk
Professor of Translational Physiology

Steven L. Britton

Nathan Qi

Lauren G. Koch

KENNETH SMITH KEN.SMITH@NOTTINGHAM.AC.UK
Professor of Metabolic Mass Spectrometry

PHILIP ATHERTON philip.atherton@nottingham.ac.uk
Professor of Clinical, metabolic & Molecular Physiology



Abstract

Introduction: Assuming myokines underlie some of the health benefits of exercise, we hypothesised that ‘high responder trainer’ (HRT) rats would exhibit distinct myokine profiles to ‘low responder trainers’ (LRT), reflecting distinct health and adaptive traits.

Methods: Blood was collected from LRT and HRT (N=8) rats at baseline (BL), immediately (0h), 1h, and 3h after running; repeated after 3-wks training. Myokines were analysed by ELISA (i.e. BDNF/Fractalkine/SPARC/Irisin/FGF21/Musclin/IL-6).

Results: At baseline, Musclin (LRT: 84 ± 24 vs HRT: 26 ± 3 pg/ml, P=0.05) and FGF21 (LRT: 133 ± 34 vs HRT: 63.5 ± 13 pg/ml, P=0.08) were higher in LRT than HRT. Training increased Musclin in HRT (26 ± 3 to 54 ± 9 pg/ml, P less than 0.05) and decreased FGF21 in LRT (133 ± 34 to 60 ± 28 pg/ml, P less than 0.05). Training increased SPARC (LRT: 0.8 ± 0.1 to 2.1 ± 0.6 ng/ml, P less than 0.05; HRT: 0.7 ± 0.06 to 1.8 ± 0.3 ng/ml, P=0.06) and Irisin (LRT 0.62 ± 0.1 to 2.6 ± 0.4 ng/ml, P less than 0.01; HRT 0.53 ± 0.1 to 2.8 ± 0.7 ng/ml, P less than 0.01) while decreasing BDNF (LRT: 2747 ± 293 to 1081 ± 330 pg/ml, P less than 0.01; HRT: 1976 ± 328 to 797 ± 160 pg/ml, P less than 0.05). Acute exercise response of Musclin (AUC) was higher in LRT vs HRT (306 ± 74 vs. 88 ± 12 pg/ml×3h-1, P less than 0.01) and elevated in HRT after training (221 ± 31 pg/ml×3h-1, P less than 0.01). Training elevated SPARC (LRT: 2.4 ± 0.1 to 7.7 ± 1.3 ng/ml×3h-1, P less than 0.05; HRT: 2.5 ± 0.13 to 11.2 ± 2.2 ng/ml×3h-1, P less than 0.001) and Irisin (LRT: 1.34 ± 0.3 to 9.6 ± 1.7 ng/ml×3h-1, P less than 0.001; HRT: 1.5 ± 0.5 to 12.1 ± 1.9 ng/ml×3h-1, P less than 0.0001).

Conclusion: Exercise training alters how myokines are secreted in response to acute exercise. Myokine responses were not robustly linked to adaptive potential in aerobic capacity, making them an unlikely regulator of adaptive traits.

Citation

Farrash, W. F., Phillips, B. E., Britton, S. L., Qi, N., Koch, L. G., Wilkinson, D. J., …Atherton, P. J. (2021). Myokine Responses to Exercise in a Rat Model of Low/High Adaptive Potential. Frontiers in Endocrinology, 12, 1-10. https://doi.org/10.3389/fendo.2021.645881

Journal Article Type Article
Acceptance Date May 17, 2021
Online Publication Date Jun 9, 2021
Publication Date Jun 9, 2021
Deposit Date Jun 4, 2021
Publicly Available Date Jun 9, 2021
Journal Frontiers in Endocrinology
Electronic ISSN 1664-2392
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 12
Article Number 6458891
Pages 1-10
DOI https://doi.org/10.3389/fendo.2021.645881
Keywords Endocrinology, Diabetes and Metabolism
Public URL https://nottingham-repository.worktribe.com/output/5624696
Publisher URL https://www.frontiersin.org/articles/10.3389/fendo.2021.645881/full

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