Biomaterials for intestinal organoid technology and personalized disease modelling

Hirota, Akira; AlMusawi, Shaikha; Nateri, Abdolrahman S.; Ordóñez-Moran, Paloma; Imajo, Masamichi

Abstract

Recent advances in intestinal organoid technologies have paved the way for in vitro recapitulation of the homeostatic renewal of adult tissues, tissue or organ morphogenesis during development, and pathogenesis of many disorders. In vitro modelling of individual patient diseases using organoid systems have been considered key in establishing rational design of personalized treatment strategies and in improving therapeutic outcomes. In addition, the transplantation of organoids into diseased tissues represents a novel approach to treat currently incurable diseases. Emerging evidence from intensive studies suggests that organoid systems’ development and functional maturation depends on the presence of an extracellular matrix with suitable biophysical properties, where advanced synthetic hydrogels open new avenues for theoretical control of organoid phenotypes and potential applications of organoids in therapeutic purposes. In this review, we discuss the status, applications, challenges and perspectives of intestinal organoid systems emphasising on hydrogels and their properties suitable for intestinal organoid culture. We provide an overview of hydrogels used for intestinal organoid culture and key factors regulating their biological activity. The comparison of different hydrogels would be a theoretical basis for establishing design principles of synthetic niches directing intestinal cell fates and functions.

Citation

Hirota, A., AlMusawi, S., Nateri, A. S., Ordóñez-Moran, P., & Imajo, M. (2021). Biomaterials for intestinal organoid technology and personalized disease modelling. Acta Biomaterialia, 132, 272-287. https://doi.org/10.1016/j.actbio.2021.05.010

• Journal Article Type: Article
• Acceptance Date: May 7, 2021
• Online Publication Date: May 20, 2021
• Publication Date: Sep 15, 2021
• Deposit Date: Jan 14, 2022
• Journal: Acta Biomaterialia
• Print ISSN: 1742-7061
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 132
• Pages: 272-287
• DOI: https://doi.org/10.1016/j.actbio.2021.05.010
• Keywords: Biotechnology; Biochemistry; Molecular Biology; Biomaterials; Biomedical Engineering; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/5569016
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1742706121003184