Mast Cell Tryptase Release Contributes to Disease Progression in Lymphangioleiomyomatosis

Babaei-Jadidi, Roya; Dongre, Arundhati; Miller, Suzanne; Castellanos Uribe, Marcos; Stewart, Iain D.; Thompson, Zoe M; Nateri, Abdolrahman S.; Bradding, Peter; May, Sean T.; Clements, Debbie; Johnson, Simon R

Abstract

Rationale: Lymphangioleiomyomatosis (LAM) is a multisystem disease that causes lung cysts and respiratory failure. Loss of TSC (tuberous sclerosis complex) gene function results in a clone of “LAM cells” with dysregulated mTOR (mechanistic target of rapamycin) activity. LAM cells and fibroblasts form lung nodules that also contain mast cells, although their significance is unknown.

Objectives: To understand the mechanism of mast-cell accumulation and the role of mast cells in the pathogenesis of LAM.

Methods: Gene expression was examined using transcriptional profiling and qRT-PCR. Mast cell/LAM nodule interactions were examined in vitro using spheroid TSC2-null cell/fibroblast cocultures and in vivo using an immunocompetent Tsc2-null murine homograft model.

Measurements and Main Results: LAM-derived cell/fibroblast cocultures induced multiple CXC chemokines in fibroblasts. LAM lungs had increased tryptase-positive mast cells expressing CXCRs (CXC chemokine receptors) (P, 0.05). Mast cells located around the periphery of LAM nodules were positively associated with the rate of lung function loss (P = 0.016). LAM spheroids attracted mast cells, and this process was inhibited by pharmacologic and CRISPR/cas9 inhibition of CXCR1 and CXCR2. LAM spheroids caused mast-cell tryptase release, which induced fibroblast proliferation and increased LAM-spheroid size (1.36 6 0.24-fold; P = 0.0019). The tryptase inhibitor APC366 and sodium cromoglycate (SCG) inhibited mast cell-induced spheroid growth. In vivo, SCG reduced mast-cell activation and Tsc2-null lung tumor burden (vehicle: 32.5.3% 6 23.6%; SCG: 5.5% 6 4.3%; P = 0.0035).

Conclusions: LAM-cell/fibroblast interactions attract mast cells where tryptase release contributes to disease progression. Repurposing SCG for use in LAM should be studied as an alternative or adjunct to mTOR inhibitor therapy.

Citation

Babaei-Jadidi, R., Dongre, A., Miller, S., Castellanos Uribe, M., Stewart, I. D., Thompson, Z. M., Nateri, A. S., Bradding, P., May, S. T., Clements, D., & Johnson, S. R. (2021). Mast Cell Tryptase Release Contributes to Disease Progression in Lymphangioleiomyomatosis. American Journal of Respiratory and Critical Care Medicine, 204(4), 431-444. https://doi.org/10.1164/rccm.202007-2854OC

• Journal Article Type: Article
• Acceptance Date: Apr 21, 2021
• Online Publication Date: Apr 21, 2021
• Publication Date: Aug 15, 2021
• Deposit Date: Jan 14, 2022
• Journal: American Journal of Respiratory and Critical Care Medicine
• Print ISSN: 1073-449X
• Electronic ISSN: 1535-4970
• Publisher: American Thoracic Society
• Peer Reviewed: Peer Reviewed
• Volume: 204
• Issue: 4
• Pages: 431-444
• DOI: https://doi.org/10.1164/rccm.202007-2854OC
• Keywords: Critical Care and Intensive Care Medicine; Pulmonary and Respiratory Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/5493703
• Publisher URL: https://www.atsjournals.org/doi/full/10.1164/rccm.202007-2854OC