Evaluating the influence of progesterone concentration and time of exposure on in vitro endometrial decidualisation

Suthaporn, Sutham; Jayaprakasan, Kanna; Thornton, Jim G.; Walker, Kate F.; Castellanos, Marcos; May, Sean; Hernandez-Medrano, Juan; Maalouf, Walid E.

Abstract

This study aimed to evaluate the influence of progesterone (concentration and time of exposure) on endometrial decidualisation using an in vitro model cell line: Human Endometrial Stromal Cells (HESCs). HESCs exposed to progesterone (1 and 10 μM) had higher percentages of decidualised cells and higher expression of the decidual marker (Insulin Like Growth Factor Binding Protein 1 (IGFBP1)) compared with those exposed to (0.1 μM). Among those HESCs cultured with 1 μM progesterone for 11 days, the highest rate of morphological differentiation (40–50%) occurred between days 7–9 and IGFBP1 peaked on day 7. The cell-cycle pathway was significantly down-regulated in HESCs exposed to at least 1 μM progesterone regardless of the incubation period. We conclude that exposure to high progesterone concentration for 7–9 days is essential to maximise the process of decidualisation.

Citation

Suthaporn, S., Jayaprakasan, K., Thornton, J. G., Walker, K. F., Castellanos, M., May, S., Hernandez-Medrano, J., & Maalouf, W. E. (2021). Evaluating the influence of progesterone concentration and time of exposure on in vitro endometrial decidualisation. Molecular and Cellular Endocrinology, 528, Article 111242. https://doi.org/10.1016/j.mce.2021.111242

• Journal Article Type: Article
• Acceptance Date: Mar 8, 2021
• Online Publication Date: Mar 11, 2021
• Publication Date: May 15, 2021
• Deposit Date: Oct 13, 2021
• Publicly Available Date: Mar 12, 2022
• Journal: Molecular and Cellular Endocrinology
• Print ISSN: 0303-7207
• Electronic ISSN: 1872-8057
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 528
• Article Number: 111242
• DOI: https://doi.org/10.1016/j.mce.2021.111242
• Public URL: https://nottingham-repository.worktribe.com/output/5481125
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0303720721000861?via%3Dihub