Dr RACHEL CLIFFORD R.Clifford@nottingham.ac.uk
ANNE MCLAREN FELLOW
TWIST1 DNA methylation is a cell marker of airway and parenchymal lung fibroblasts that are differentially methylated in asthma
Clifford, Rachel L.; Yang, Chen Xi; Fishbane, Nick; Patel, Jamie; MacIsaac, Julia L.; McEwen, Lisa M.; May, Sean T.; Castellanos-Uribe, Marcos; Nair, Parameswaran; Obeidat, Ma�en; Kobor, Michael S.; Knox, Alan J.; Hackett, Tillie Louise
Authors
Chen Xi Yang
Nick Fishbane
Jamie Patel
Julia L. MacIsaac
Lisa M. McEwen
Professor SEAN MAY SEAN.MAY@NOTTINGHAM.AC.UK
PROFESSOR OF PLANT CYBER INFRASTRUCTURE
Marcos Castellanos-Uribe
Parameswaran Nair
Ma�en Obeidat
Michael S. Kobor
Alan J. Knox
Tillie Louise Hackett
Abstract
© 2020 The Author(s). Background: Mesenchymal fibroblasts are ubiquitous cells that maintain the extracellular matrix of organs. Within the lung, airway and parenchymal fibroblasts are crucial for lung development and are altered with disease, but it has been difficult to understand their roles due to the lack of distinct molecular markers. We studied genome-wide DNA methylation and gene expression in airway and parenchymal lung fibroblasts from healthy and asthmatic donors, to identify a robust cell marker and to determine if these cells are molecularly distinct in asthma. Results: Airway (N = 8) and parenchymal (N = 15) lung fibroblasts from healthy individuals differed in the expression of 158 genes, and DNA methylation of 3936 CpGs (Bonferroni adjusted p value < 0.05). Differential DNA methylation between cell types was associated with differential expression of 42 genes, but no single DNA methylation CpG feature (location, effect size, number) defined the interaction. Replication of gene expression and DNA methylation in a second cohort identified TWIST1 gene expression, DNA methylation and protein expression as a cell marker of airway and parenchymal lung fibroblasts, with DNA methylation having 100% predictive discriminatory power. DNA methylation was differentially altered in parenchymal (112 regions) and airway fibroblasts (17 regions) with asthmatic status, with no overlap between regions. Conclusions: Differential methylation of TWIST1 is a robust cell marker of airway and parenchymal lung fibroblasts. Airway and parenchymal fibroblast DNA methylation are differentially altered in individuals with asthma, and the role of both cell types should be considered in the pathogenesis of asthma.
Citation
Clifford, R. L., Yang, C. X., Fishbane, N., Patel, J., MacIsaac, J. L., McEwen, L. M., May, S. T., Castellanos-Uribe, M., Nair, P., Obeidat, M., Kobor, M. S., Knox, A. J., & Hackett, T. L. (2020). TWIST1 DNA methylation is a cell marker of airway and parenchymal lung fibroblasts that are differentially methylated in asthma. Clinical Epigenetics, 12(1), Article 145. https://doi.org/10.1186/s13148-020-00931-4
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 1, 2020 |
Online Publication Date | Oct 2, 2020 |
Publication Date | 2020-12 |
Deposit Date | Oct 16, 2020 |
Publicly Available Date | Oct 16, 2020 |
Journal | Clinical Epigenetics |
Print ISSN | 1868-7075 |
Electronic ISSN | 1868-7083 |
Publisher | Springer Verlag |
Peer Reviewed | Peer Reviewed |
Volume | 12 |
Issue | 1 |
Article Number | 145 |
DOI | https://doi.org/10.1186/s13148-020-00931-4 |
Keywords | Genetics(clinical); Developmental Biology; Genetics; Molecular Biology |
Public URL | https://nottingham-repository.worktribe.com/output/4939107 |
Publisher URL | https://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/s13148-020-00931-4 |
Files
s13148-020-00931-4
(4.3 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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