Application of a novel phage LPSEYT for biological control of Salmonella in foods

Yan, Ting; Liang, Lu; Yin, Ping; Zhou, Yang; Sharoba, Ashraf Mahdy; Lu, Qun; Dong, Xingxing; Liu, Kun; Connerton, Ian F.; Li, Jinquan

Abstract

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Salmonella is a leading cause of foodborne diseases, and in recent years, many isolates have exhibited a high level of antibiotic resistance, which has led to huge pressures on public health. Phages are a promising strategy to control food‐borne pathogens. In this study, one of our environmental phage isolates, LPSEYT, was to be able to restrict the growth of zoonotic Salmonella enterica in vitro over a range of multiplicity of infections. Phage LPSEYT exhibited wide‐ranging pH and thermal stability and rapid reproductive activity with a short latent period and a large burst size. Phage LPSEYT demonstrated potential efficiency as a biological control agent against Salmonella in a variety of food matrices, including milk and lettuce. Morphological observation, comparative genomic, and phylogenetic analysis revealed that LPSEYT does not belong to any of the currently identified genera within the Myoviridae family, and we suggest that LPSEYT represents a new genus, the LPSEYTvirus. This study contributes a phage database, develops beneficial phage resources, and sheds light on the potential application value of phages LPSEYT on food safety.

Citation

Yan, T., Liang, L., Yin, P., Zhou, Y., Sharoba, A. M., Lu, Q., Dong, X., Liu, K., Connerton, I. F., & Li, J. (2020). Application of a novel phage LPSEYT for biological control of Salmonella in foods. Microorganisms, 8(3), Article 400. https://doi.org/10.3390/microorganisms8030400

• Journal Article Type: Article
• Acceptance Date: Mar 10, 2020
• Online Publication Date: Mar 12, 2020
• Publication Date: Mar 1, 2020
• Deposit Date: Mar 11, 2020
• Publicly Available Date: Mar 16, 2020
• Journal: Microorganisms
• Electronic ISSN: 2076-2607
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 3
• Article Number: 400
• DOI: https://doi.org/10.3390/microorganisms8030400
• Public URL: https://nottingham-repository.worktribe.com/output/4126966
• Publisher URL: https://www.mdpi.com/2076-2607/8/3/400