Quantification of GABA, glutamate and glutamine in a single measurement at 3 T using GABA-edited MEGA-PRESS

Nezhad, Faezeh Sanaei; Anton, Adriana; Michou, Emilia; Jung, JeYoung; Parkes, Laura M.; Williams, Stephen R.

Abstract

γ-Aminobutyric acid (GABA) and glutamate (Glu), major neurotransmitters in the brain, are recycled through glutamine (Gln). All three metabolites can be measured by magnetic resonance spectroscopy in vivo, although GABA measurement at 3T requires an extra editing acquisition, such as Mescher–Garwood point-resolved spectroscopy (MEGA-PRESS). In a GABA-edited MEGA-PRESS spectrum, Glu and Gln co-edit with GABA, providing the possibility to measure all three in one acquisition. In this study, we investigated the reliability of the composite Glu+Gln (Glx) peak estimation and the possibility of Glu and Gln separation in GABA-edited MEGA-PRESS spectra. The data acquired in vivo were used to develop a quality assessment framework which identified MEGA-PRESS spectra in which Glu and Gln could be estimated reliably. Phantoms containing Glu, Gln, GABA and N-acetylaspartate (NAA) at different concentrations were scanned using GABA-edited MEGA-PRESS at 3T. Fifty-six sets of spectra in five brain regions were acquired from 36 healthy volunteers. Based on the Glu/Gln ratio, data were classified as either within or outside the physiological range. A peak-by-peak quality assessment was performed on all data to investigate whether quality metrics can discriminate between these two classes of spectra. The quality metrics were as follows: the GABA signal-to-noise ratio, the NAA linewidth and the Glx Cramer–Rao lower bound (CRLB). The Glu and Gln concentrations were estimated with precision across all phantoms with a linear relationship between the measured and true concentrations: R1 = 0.95 for Glu and R1 = 0.91 for Gln. A quality assessment framework was set based on the criteria necessary for a good GABA-edited MEGA-PRESS spectrum. Simultaneous criteria of NAA linewidth <8Hz and Glx CRLB <16% were defined as optimum features for reliable Glu and Gln quantification. Glu and Gln can be reliably quantified from GABA-edited MEGA-PRESS acquisitions. However, this reliability should be controlled using the quality assessment methods suggested in this work.

Citation

Nezhad, F. S., Anton, A., Michou, E., Jung, J., Parkes, L. M., & Williams, S. R. (2018). Quantification of GABA, glutamate and glutamine in a single measurement at 3 T using GABA-edited MEGA-PRESS. NMR in Biomedicine, 31(1), Article e3847. https://doi.org/10.1002/nbm.3847

• Journal Article Type: Article
• Acceptance Date: Sep 19, 2017
• Online Publication Date: Nov 12, 2017
• Publication Date: 2018-01
• Deposit Date: May 22, 2020
• Publicly Available Date: Jun 5, 2020
• Journal: NMR in Biomedicine
• Print ISSN: 0952-3480
• Electronic ISSN: 1099-1492
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 31
• Issue: 1
• Article Number: e3847
• DOI: https://doi.org/10.1002/nbm.3847
• Public URL: https://nottingham-repository.worktribe.com/output/3952352
• Publisher URL: https://onlinelibrary.wiley.com/doi/full/10.1002/nbm.3847