Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens

Morra, Simone; Valetti, Francesca; Sarasso, Veronica; Castrignanò, Silvia; Sadeghi, Sheila J.; Gilardi, Gianfranco

Abstract

© 2015 Elsevier B.V. The [FeFe]-hydrogenase CpHydA from Clostridium perfringens was immobilized by adsorption on anatase TiO2 electrodes for clean hydrogen production. The immobilized enzyme proved to perform direct electron transfer to and from the electrode surface and catalyses both H2 oxidation (H2 uptake) and H2 production (H2 evolution) with a current density for H2 evolution of about 2mAcm-1. The TiO2/CpHydA bioelectrode remained active for several days upon storage and when a reducing potential was set, H2 evolution occurred with a mean Faradaic efficiency of 98%. The high turnover frequency of H2 production and the tight coupling of electron transfer, resulting in a Faradaic efficiency close to 100%, support the exploitation of the novel TiO2/CpHydA stationary electrode as a powerful device for H2 production.

Citation

Morra, S., Valetti, F., Sarasso, V., Castrignanò, S., Sadeghi, S. J., & Gilardi, G. (2015). Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens. Bioelectrochemistry, 106, 258-262. https://doi.org/10.1016/j.bioelechem.2015.08.001

• Journal Article Type: Article
• Acceptance Date: Aug 7, 2015
• Online Publication Date: Aug 11, 2015
• Publication Date: 2015-12
• Deposit Date: Jan 31, 2020
• Publicly Available Date: Feb 11, 2020
• Journal: Bioelectrochemistry
• Print ISSN: 1567-5394
• Electronic ISSN: 1878-562X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 106
• Pages: 258-262
• DOI: https://doi.org/10.1016/j.bioelechem.2015.08.001
• Public URL: https://nottingham-repository.worktribe.com/output/3841456
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S1567539415300177?via%3Dihub
• Additional Information: This article is maintained by: Elsevier; Article Title: Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens; Journal Title: Bioelectrochemistry; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.bioelechem.2015.08.001; Content Type: article; Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.