The effect of hyperglycemia on neurovascular coupling and cerebrovascular patterning in zebrafish

Chhabria, Karishma; Plant, Karen; Bandmann, Oliver; Wilkinson, Robert N; Martin, Chris; Kugler, Elisabeth; Armitage, Paul A; Santoscoy, Paola LM; Cunliffe, Vincent T; Huisken, Jan; McGown, Alexander; Ramesh, Tennore; Chico, Tim JA; Howarth, Clare

Abstract

Neurovascular coupling (through which local cerebral blood flow changes in response to neural activation are mediated) is impaired in many diseases including diabetes. Current preclinical rodent models of neurovascular coupling rely on invasive surgery and instrumentation, but transgenic zebrafish coupled with advances in imaging techniques allow non-invasive quantification of cerebrovascular anatomy, neural activation, and cerebral vessel haemodynamics. We therefore established a novel non-invasive, non-anaesthetised zebrafish larval model of neurovascular coupling, in which visual stimulus evokes neuronal activation in the optic tectum that is associated with a specific increase in red blood cell speed in tectal blood vessels. We applied this model to the examination of the effect of glucose exposure on cerebrovascular patterning and neurovascular coupling. We found that chronic exposure of zebrafish to glucose impaired tectal blood vessel patterning and neurovascular coupling. The nitric oxide donor sodium nitroprusside rescued all these adverse effects of glucose exposure on cerebrovascular patterning and function. Our results establish the first non-mammalian model of neurovascular coupling, offering the potential to perform more rapid genetic modifications and high-throughput screening than is currently possible using rodents. Furthermore, using this zebrafish model, we reveal a potential strategy to ameliorate the effects of hyperglycemia on cerebrovascular function

Citation

Chhabria, K., Plant, K., Bandmann, O., Wilkinson, R. N., Martin, C., Kugler, E., Armitage, P. A., Santoscoy, P. L., Cunliffe, V. T., Huisken, J., McGown, A., Ramesh, T., Chico, T. J., & Howarth, C. (2020). The effect of hyperglycemia on neurovascular coupling and cerebrovascular patterning in zebrafish. Journal of Cerebral Blood Flow and Metabolism, 40(2), 298-313. https://doi.org/10.1177/0271678X18810615

• Journal Article Type: Article
• Acceptance Date: Oct 2, 2018
• Online Publication Date: Nov 6, 2018
• Publication Date: 2020-02
• Deposit Date: Nov 12, 2019
• Publicly Available Date: Jan 15, 2020
• Journal: Journal of Cerebral Blood Flow & Metabolism
• Print ISSN: 0271-678X
• Electronic ISSN: 1559-7016
• Publisher: SAGE Publications
• Peer Reviewed: Peer Reviewed
• Volume: 40
• Issue: 2
• Pages: 298-313
• DOI: https://doi.org/10.1177/0271678X18810615
• Public URL: https://nottingham-repository.worktribe.com/output/3231091
• Publisher URL: https://journals.sagepub.com/doi/10.1177/0271678X18810615