Coexpression of gastrin and gastrin receptors (CCK-B and ΔCCK-B) in gastrointestinal tumour cell lines

McWilliams, D. F.; Watson, S. A.; Crosbee, D. M.; Michaeli, D.; Seth, R.

Abstract

Background - The peptide hormone gastrin is a recognised growth factor for gastrointestinal (GI) tumour cells. Carboxyamidated gastrins bind to the cell surface gastrin/cholecystokinin B (CCK-B) receptor which can be expressed as either a normal or a truncated isoform (ΔCCK-B). Aims - To compare gastrin gene expression with ΔCCK-B and total CCK-B (both isoforms) gene expression in both GI and non-GI tract derived human tumour cell lines. Methods - Total RNA was extracted and gene expression was assayed by the reverse transcription-polymerase chain reaction followed by Southern blotting and hybridisation with specific oligo probes. Results - Gastrin was expressed by 5/5 gastric and 7/8 colorectal cell lines. Coexpression of gastrin CCK-B isoform was found in 80% of gastric and 75% of colorectal cell lines. Non-GI cell lines, with the exception of a lymphoblastic leukaemia cell line, showed no coexpression. The truncated receptor, ΔCCK-B, was shown in 3/5 gastric and 5/8 colorectal cell lines and was always coexpressed with gastrin. Conclusion - The truncated gastrin receptor, ΔCCK-B, is coexpressed with gastrin in 8/13 GI tumour cell lines. Gastrin and CCK-B receptor isoforms may be involved in maintaining autocrine/paracrine growth pathways in GI cancer cells.

Citation

McWilliams, D. F., Watson, S. A., Crosbee, D. M., Michaeli, D., & Seth, R. (1998). Coexpression of gastrin and gastrin receptors (CCK-B and ΔCCK-B) in gastrointestinal tumour cell lines. Gut, 42(6), 795-798. https://doi.org/10.1136/gut.42.6.795

• Journal Article Type: Article
• Acceptance Date: Jan 19, 1998
• Online Publication Date: Jun 1, 1998
• Publication Date: Jun 1, 1998
• Deposit Date: Jul 31, 2023
• Journal: Gut
• Print ISSN: 0017-5749
• Electronic ISSN: 1468-3288
• Publisher: BMJ Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 42
• Issue: 6
• Pages: 795-798
• DOI: https://doi.org/10.1136/gut.42.6.795
• Public URL: https://nottingham-repository.worktribe.com/output/3204314
• Publisher URL: https://gut.bmj.com/content/42/6/795