Antigenicity of chimeric and cyclic synthetic peptides based on nonstructural proteins of GBV-C/HGV

P�rez, T.; Ercilla, G.; Chan, W. C.; Haro, I.

Abstract

In this work, new putative epitopes located in nonstructural proteins of GBV-C/HGV were synthesized using solid-phase chemistry for their use in immunoassays. The antigens were obtained in linear, chimeric and cyclic forms with the main aim of improving the sensitivity of the enzyme immunoassays. Our results showed, on one hand, that the combination of different antigens seems to be necessary to ensure good sensitivity and more specificity and, on the other hand, that cyclic compounds show higher ability to recognize anti-GBV-C/HGV antibodies than its parent peptide. Furthermore, CD and MR have been used in conjunction to characterize the conformational changes therein with synthetic constructs that could explain their different antigenicity. Copyright © 2005 European Peptide Society and John Wiley & Sons, Ltd.

Citation

Pérez, T., Ercilla, G., Chan, W. C., & Haro, I. (2006). Antigenicity of chimeric and cyclic synthetic peptides based on nonstructural proteins of GBV-C/HGV. Journal of Peptide Science, 12(4), 267-278. https://doi.org/10.1002/psc.719

• Journal Article Type: Article
• Acceptance Date: Jul 11, 2005
• Online Publication Date: Sep 23, 2005
• Publication Date: Apr 1, 2006
• Deposit Date: Sep 10, 2020
• Journal: Journal of Peptide Science
• Print ISSN: 1075-2617
• Electronic ISSN: 1099-1387
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 4
• Pages: 267-278
• DOI: https://doi.org/10.1002/psc.719
• Public URL: https://nottingham-repository.worktribe.com/output/3137737
• Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/psc.719