Quantification of Fluorescent Reporters in Plant Cells

Pound, Michael; French, Andrew P.; Wells, Darren M.

Abstract

© Springer Science+Business Media New York 2015. Fluorescent reporters are powerful tools for plant research. Many studies require accurate determination of fluorescence intensity and localization. Here, we describe protocols for the quantification of fluorescence intensity in plant cells from confocal laser scanning microscope images using semiautomated software and image analysis techniques.

• Acceptance Date: Oct 1, 2014
• Online Publication Date: Oct 30, 2014
• Publication Date: 2015
• Deposit Date: Feb 18, 2020
• Publisher: Springer
• Pages: 123-131
• Series Title: Methods in Molecular Biology
• Series Number: 1242
• Book Title: Plant Cell Expansion: Methods and Protocols
• ISBN: 9781493919017
• DOI: https://doi.org/10.1007/978-1-4939-1902-4_11
• Public URL: https://nottingham-repository.worktribe.com/output/3125121
• Publisher URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-1902-4_11