Claire Horlock
Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristics
Horlock, Claire; Shakib, Farouk; Mahdavi, Jafar; Jones, Nick S.; Sewell, Herb F.; Ghaemmaghami, Amir M.
Authors
Farouk Shakib
Jafar Mahdavi
Nick S. Jones
Herb F. Sewell
Professor AMIR GHAEMMAGHAMI AMIR.GHAEMMAGHAMI@NOTTINGHAM.AC.UK
Professor of Immunology and Immuno- Bioengineering
Abstract
Background: Dendritic cells (DCs) are specialized antigen presenting cells that play a pivotal role in bridging innate and adaptive immune responses. Given the scarcity of peripheral blood myeloid dendritic cells (mDCs) investigators have used different model systems for studying DC biology. Monocyte-derived dendritic cells (moDCs) and KG-1 cells are routinely used as mDC models, but a thorough comparison of these cells has not yet been carried out, particularly in relation to their proteomes. We therefore sought to run a comparative study of the proteomes and functional properties of these cells. Results: Despite general similarities between mDCs and the model systems, moDCs and KG-1 cells, our findings identified some significant differences in the proteomes of these cells, and the findings were confirmed by ELISA detection of a selection of proteins. This was particularly noticeable with proteins involved in cell growth and maintenance (for example, fibrinogen γ chain (FGG) and ubiquinol cytochrome c) and cell-cell interaction and integrity (for example, fascin and actin). We then examined the surface phenotype, cytokine profile, endocytic and T-cell-activation ability of these cells in support of the proteomic data, and obtained confirmatory evidence for differences in the maturation status and functional attributes between mDCs and the two DC models. Conclusion: We have identified important proteomic and functional differences between mDCs and two DC model systems. These differences could have major functional implications, particularly in relation to DC-T cell interactions, the so-called immunological synapse, and, therefore, need to be considered when interpreting data obtained from model DC systems. © 2007 Horlock et al.; licensee BioMed Central Ltd.
Citation
Horlock, C., Shakib, F., Mahdavi, J., Jones, N. S., Sewell, H. F., & Ghaemmaghami, A. M. (2007). Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristics. Genome Biology, 8(3), Article R30. https://doi.org/10.1186/gb-2007-8-3-r30
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 1, 2007 |
Online Publication Date | Mar 1, 2007 |
Publication Date | Mar 1, 2007 |
Deposit Date | Jan 3, 2023 |
Publicly Available Date | Jan 20, 2023 |
Journal | Genome Biology |
Print ISSN | 1474-760X |
Electronic ISSN | 1465-6906 |
Publisher | Springer Verlag |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 3 |
Article Number | R30 |
DOI | https://doi.org/10.1186/gb-2007-8-3-r30 |
Public URL | https://nottingham-repository.worktribe.com/output/3097612 |
Publisher URL | https://genomebiology.biomedcentral.com/articles/10.1186/gb-2007-8-3-r30 |
Files
gb-2007-8-3-r30
(3.7 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/2.0/
You might also like
Investigating NF-kappa B signaling in lung fibroblasts in 2D and 3D culture systems
(2015)
Journal Article
Allergen recognition by innate immune cells: critical role of dendritic and epithelial cells
(2013)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search