Rhys C. Griffiths
Peptide Conjugation: The Chemical Synthesis of Site-Specifically Modified Proteins via Diselenide-Selenoester Ligation
Griffiths, Rhys C.; Mitchell, NIcholas J.
Authors
Dr NICHOLAS MITCHELL NICHOLAS.MITCHELL@NOTTINGHAM.AC.UK
Associate Professor
Contributors
Waleed M. Hussein
Editor
Rachel J. Stephenson
Editor
Istvan Toth
Editor
Abstract
Peptide ligation techniques enable the controlled chemical synthesis of native and engineered proteins, including examples that display site-specific post-translational modifications (PTMs) and non-proteinogenic functionality. Diselenide-selenoester ligation (DSL) is a recent addition to the synthetic methodology that offers several advantages over existing strategies. The standard DSL reaction involves the additive-free ligation of a peptide carrying an N-terminal selenocysteine (Sec) residue with a fragment bearing a C-terminal selenoester. This operationally simple ligation proceeds rapidly at sterically hindered junctions and is efficient across a broad pH range. The incorporation of deselenization and oxidative deselenization techniques into the DSL protocol enables conversion of the Sec residue at the ligation site to alanine (Ala) and serine (Ser), respectively, thus enhancing the scope and versatility of the method. In this chapter, we describe the application of DSL to the one-pot chemical synthesis of proteins via both two-component and three-component ligation pathways.
Citation
Griffiths, R. C., & Mitchell, N. J. (2021). Peptide Conjugation: The Chemical Synthesis of Site-Specifically Modified Proteins via Diselenide-Selenoester Ligation. In W. M. Hussein, R. J. Stephenson, & I. Toth (Eds.), Peptide Conjugation: Methods and Protocols (231-251). Humana Press. https://doi.org/10.1007/978-1-0716-1617-8
Online Publication Date | Aug 13, 2021 |
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Publication Date | 2021 |
Deposit Date | Jan 13, 2024 |
Publisher | Humana Press |
Pages | 231-251 |
Series Title | Methods in Molecular Biology |
Series Number | 2355 |
Book Title | Peptide Conjugation: Methods and Protocols |
ISBN | 9781071616161 |
DOI | https://doi.org/10.1007/978-1-0716-1617-8 |
Public URL | https://nottingham-repository.worktribe.com/output/29555985 |
Publisher URL | https://link.springer.com/protocol/10.1007/978-1-0716-1617-8_18 |
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