Rhys C. Griffiths
Site‐Selective Installation of Nϵ ‐Modified Sidechains into Peptide and Protein Scaffolds via Visible‐Light‐Mediated Desulfurative C–C Bond Formation
Griffiths, Rhys C.; Smith, Frances R.; Long, Jed E.; Scott, Daniel; Williams, Huw E. L.; Oldham, Neil J.; Layfield, Robert; Mitchell, Nicholas J.
Authors
Frances R. Smith
Jed E. Long
Dr Daniel Scott DANIEL.SCOTT@NOTTINGHAM.AC.UK
NOTTINGHAM RESEARCH FELLOW
Dr HUW WILLIAMS HUW.WILLIAMS@NOTTINGHAM.AC.UK
SENIOR RESEARCH FELLOW
Professor NEIL OLDHAM NEIL.OLDHAM@NOTTINGHAM.AC.UK
PROFESSOR OF BIOMOLECULAR SPECTROMETRY
Professor Rob Layfield ROBERT.LAYFIELD@NOTTINGHAM.AC.UK
PROFESSOR OF PROTEIN BIOCHEMISTRY
Dr NICHOLAS MITCHELL NICHOLAS.MITCHELL@NOTTINGHAM.AC.UK
Associate Professor
Abstract
Post-translational modifications (PTMs) enhance the repertoire of protein function and mediate or influence the activity of many cellular processes. The preparation of site-specifically and homogeneously modified proteins, to apply as tools to understand the biological role of PTMs, is a challenging task. Herein, we describe a visible-light-mediated desulfurative C(sp3)–C(sp3) bond forming reaction that enables the site-selective installation of Nϵ-modified sidechains into peptides and proteins of interest. Rapid, operationally simple, and tolerant to ambient atmosphere, we demonstrate the installation of a range of lysine (Lys) PTMs into model peptide systems and showcase the potential of this technology by site-selectively installing an NϵAc sidechain into recombinantly expressed ubiquitin (Ub).
Citation
Griffiths, R. C., Smith, F. R., Long, J. E., Scott, D., Williams, H. E. L., Oldham, N. J., Layfield, R., & Mitchell, N. J. (2022). Site‐Selective Installation of Nϵ ‐Modified Sidechains into Peptide and Protein Scaffolds via Visible‐Light‐Mediated Desulfurative C–C Bond Formation. Angewandte Chemie, 134(2), Article e202110223. https://doi.org/10.1002/ange.202110223
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Oct 29, 2021 |
| Online Publication Date | Dec 3, 2021 |
| Publication Date | Jan 10, 2022 |
| Deposit Date | Aug 22, 2022 |
| Journal | Angewandte Chemie |
| Print ISSN | 0044-8249 |
| Electronic ISSN | 1521-3757 |
| Publisher | Wiley |
| Peer Reviewed | Peer Reviewed |
| Volume | 134 |
| Issue | 2 |
| Article Number | e202110223 |
| DOI | https://doi.org/10.1002/ange.202110223 |
| Keywords | General Medicine |
| Public URL | https://nottingham-repository.worktribe.com/output/7511319 |
| Publisher URL | https://onlinelibrary.wiley.com/doi/10.1002/ange.202110223 |
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