Molecular characterisation of defence of Brassica napus (Oilseed rape) to Rhizoctonia solani AG2-1 confirmed by functional analysis in Arabidopsis thaliana

Sims, Isabelle; Jayaweera, Dasuni; Swarup, Kamal; Ray, Rumiana V.

Abstract

Rhizoctonia solani is a necrotrophic, soilborne fungal pathogen associated with significant establishment losses in Brassica napus (oilseed rape; OSR). The anastomosis group (AG) 2-1 of R. solani is the most virulent to OSR, causing damping-off, root and hypocotyl rot, and seedling death. Resistance to R. solani AG2-1 in OSR has not been identified, and the regulation of OSR defense to its adapted pathogen, AG2-1, has not been investigated. In this work, we used confocal microscopy to visualize the progress of infection by sclerotia of AG2-1 on B. napus varieties with contrasting disease phenotypes. We defined their defense response using gene expression studies and functional analysis with Arabidopsis thaliana mutants. Our results showed existing variation in susceptibility to AG2-1 and plant growth between OSR varieties, and differential expression of genes of hormonal and defense pathways related to auxin, ethylene, jasmonic acid, abscisic acid, salicylic acid, and reactive oxygen species regulation. Auxin, abscisic acid signaling, and the MYC2 branch of jasmonate signaling contributed to the susceptibility to AG2-1, while induced systemic resistance was enhanced by NAPDH RBOHD, ethylene signaling, and the ERF/PDF branch of jasmonate signaling. These results pave the way for future research, which will lead to the development of Brassica crops that are more resistant to AG2-1 of R. solani and reduce dependence on chemical control options.

Citation

Sims, I., Jayaweera, D., Swarup, K., & Ray, R. V. (2023). Molecular characterisation of defence of Brassica napus (Oilseed rape) to Rhizoctonia solani AG2-1 confirmed by functional analysis in Arabidopsis thaliana. Phytopathology, 113(8), 1525-1536. https://doi.org/10.1094/PHYTO-08-22-0305-R

• Journal Article Type: Article
• Acceptance Date: Mar 13, 2023
• Online Publication Date: Oct 3, 2023
• Publication Date: 2023-08
• Deposit Date: Jan 26, 2024
• Publicly Available Date: Jan 30, 2024
• Journal: Phytopathology
• Print ISSN: 0031-949X
• Electronic ISSN: 1943-7684
• Publisher: American Phytopathological Society
• Peer Reviewed: Peer Reviewed
• Volume: 113
• Issue: 8
• Pages: 1525-1536
• DOI: https://doi.org/10.1094/PHYTO-08-22-0305-R
• Keywords: Plant Immune Responses, Disease Control and Pest Management, Molecular, Disease Resistance, Host Parasite Interactions, Fungal Pathogens
• Public URL: https://nottingham-repository.worktribe.com/output/19287061
• Publisher URL: ttps://apsjournals.apsnet.org/doi/10.1094/PHYTO-08-22-0305-R