The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway

Zhang, Xuebin; Jayaweera, Dasuni; Peters, Janny L.; Szecsi, Judit; Bendahmane, Mohammed; Roberts, Jeremy A.; Gonzalez-Carranza, Zinnia H.

Abstract

In Arabidopsis, the F-box HAWAIIAN SKIRT (HWS) protein is important for organ growth. Loss of function of HWS exhibits pleiotropic phenotypes including sepal fusion. To dissect the HWS role, we EMS-mutagenized hws-1 seeds and screened for mutations that suppress hws-1 associated phenotypes. We identified shs-2 and shs-3 (suppressor of hws-2 and 3) mutants in which the sepal fusion phenotype of hws-1 was suppressed. shs-2 and shs-3 (renamed hst-23/hws-1 and hst-24/hws-1) carry transition mutations that result in premature terminations in the plant homolog of Exportin-5 HASTY (HST), known to be important in miRNA biogenesis, function and transport. Genetic crosses between hws-1 and mutant lines for genes in the miRNA pathway, also suppress the phenotypes associated with HWS loss of function, corroborating epistatic relations between the miRNA pathway genes and HWS. In agreement with these data, accumulation of miRNA is modified in HWS loss or gain of function mutants. Our data propose HWS as a new player in the miRNA pathway, important for plant growth.

Citation

Zhang, X., Jayaweera, D., Peters, J. L., Szecsi, J., Bendahmane, M., Roberts, J. A., & Gonzalez-Carranza, Z. H. (2017). The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway. PLoS ONE, 12(12), Article e0189788. https://doi.org/10.1371/journal.pone.0189788

• Journal Article Type: Article
• Acceptance Date: Dec 8, 2017
• Publication Date: Dec 15, 2017
• Deposit Date: Dec 11, 2017
• Publicly Available Date: Dec 15, 2017
• Journal: PLoS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 12
• Article Number: e0189788
• DOI: https://doi.org/10.1371/journal.pone.0189788
• Public URL: https://nottingham-repository.worktribe.com/output/900291
• Publisher URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0189788
• Contract Date: Dec 11, 2017