Dean Walsh
The biocide triclosan induces (p)ppGpp dependent antibiotic tolerance and alters SarA dependent biofilm structures in Staphylococcus aureus
Walsh, Dean; Salzer, Andrea; Wolz, Christiane; Aylott, Jonathan; Hardie, Kim Rachael
Authors
Andrea Salzer
Christiane Wolz
Professor JONATHAN AYLOTT JON.AYLOTT@NOTTINGHAM.AC.UK
PROFESSOR OF ANALYTICAL SCIENCE
Professor KIM HARDIE KIM.HARDIE@NOTTINGHAM.AC.UK
PROFESSOR OF BACTERIAL PATHOGENESIS
Abstract
The biocide triclosan is used extensively in both household and hospital settings. The chronic exposure to the biocide occurring in individuals that use triclosan-containing products results in low levels of triclosan present in the human body that has been linked to induction of antibiotic tolerance and altered biofilm formation. Here we aimed to unravel the molecular mechanisms involved in triclosan induced antibiotic tolerance and biofilm formation in Staphylococcus aureus. Triclosan treatment prior to planktonic exposure to bactericidal antibiotics resulted in 1,000 fold higher viable cell counts compared to non-pretreated cultures. Triclosan pretreatment also protected S. aureus biofilms against otherwise lethal doses of antibiotics as shown by live/dead cell staining and viable cell counting. Triclosan mediated antibiotic tolerance in planktonic and biofilm cultures required an active stringent response because a pppGpp0 strain was not protected from antibiotic killing. Incubation of S. aureus with triclosan also altered biofilm structure due to SarA-mediated overproduction of the polysaccharide intercellular adhesin (PIA) in the biofilm matrix. Thus, physiologically relevant concentrations of triclosan can trigger (p)ppGpp dependent antibiotic tolerance as well as SarA dependent biofilm formation.
Citation
Walsh, D., Salzer, A., Wolz, C., Aylott, J., & Hardie, K. R. The biocide triclosan induces (p)ppGpp dependent antibiotic tolerance and alters SarA dependent biofilm structures in Staphylococcus aureus
Working Paper Type | Working Paper |
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Deposit Date | Feb 3, 2023 |
Publicly Available Date | Feb 7, 2023 |
Public URL | https://nottingham-repository.worktribe.com/output/16802336 |
Publisher URL | https://www.biorxiv.org/content/10.1101/2023.02.01.525840v1 |
Files
(p)ppGpp dependent antibiotic tolerance
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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