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Myoinositol CEST signal in animals with increased Iba-1 levels in response to an inflammatory challenge—Preliminary findings

Yanez Lopez, Maria; Pardon, Marie-Christine; Baiker, Kerstin; Prior, Malcolm; Yuchun, Ding; Agostini, Alessandra; Bai, Li; Auer, Dorothee P.; Faas, Henryk M.

Authors

Maria Yanez Lopez

Ding Yuchun

Alessandra Agostini

Li Bai

Henryk M. Faas



Abstract

Neuroinflammation plays an important role in the pathogenesis of a range of brain disorders. Non-invasive imaging of neuroinflammation is critical to help improve our understanding of the underlying disease mechanisms, monitor therapies and guide drug development. Generally, MRI lacks specificity to molecular imaging biomarkers, but molecular MR imaging based on chemical exchange saturation transfer (CEST) can potentially detect changes of myoinositol, a putative glial marker that may index neuroinflammation. In this pilot study we aimed to investigate, through validation with immunohistochemistry and in vivo magnetic resonance spectroscopy (MRS), whether CEST imaging can reflect the microglial response to a mild inflammatory challenge with lipopolysaccharide (LPS), in the APPSwe/ PS1 mouse model of Alzheimer’s disease and wild type controls. The response to the immune challenge was variable and did not align with genotype. Animals with a strong response to LPS (Iba1+, n = 6) showed an increase in CEST contrast compared with those who did not (Iba1-, n = 6). Changes of myoinositol levels after LPS were not significant. We discuss the difficulties of this mild inflammatory model, the role of myoinositol as a glial biomarker, and the technical challenges of CEST imaging at 0.6ppm.

Journal Article Type Article
Publication Date Feb 21, 2019
Journal PLOS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 14
Issue 2
Article Number e0212002
APA6 Citation Yanez Lopez, M., Pardon, M., Baiker, K., Prior, M., Yuchun, D., Agostini, A., …Faas, H. M. (2019). Myoinositol CEST signal in animals with increased Iba-1 levels in response to an inflammatory challenge—Preliminary findings. PLoS ONE, 14(2), https://doi.org/10.1371/journal.pone.0212002
DOI https://doi.org/10.1371/journal.pone.0212002
Keywords General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences; General Medicine
Additional Information Yanez Lopez M, Pardon M-C, Baiker K, Prior M, Yuchun D, Agostini A, et al. (2019) Myoinositol CEST signal in animals with increased Iba-1 levels in response to an inflammatory challenge—Preliminary findings. PLoS ONE 14(2): e0212002. https://doi.org/10.1371/journal.pone.0212002

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