Benedetta Guidi
Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form
Guidi, Benedetta; Planchestainer, Matteo; Contente, Martina Letizia; Laurenzi, Tommaso; Eberini, Ivano; Gourlay, Louise J.; Romano, Diego; Paradisi, Francesca; Molinari, Francesco
Authors
Matteo Planchestainer
MARTINA CONTENTE MARTINA.CONTENTE@NOTTINGHAM.AC.UK
Research Fellow in Enzymeimmobilization and Flow-Biocatalysis
Tommaso Laurenzi
Ivano Eberini
Louise J. Gourlay
Diego Romano
FRANCESCA PARADISI FRANCESCA.PARADISI@NOTTINGHAM.AC.UK
Professor of Biocatalysis
Francesco Molinari
Abstract
A new transaminase (VbTA) was identified from the genome of the halotolerant marine bacterium Virgibacillus 21D and its expression resulted in the production of an insoluble protein in E. coli. After a single mutation identified through sequence homology analysis, the mutant VbTA T16F was successfully expressed as a recombinant protein and characterised. VbTA T16F showed high stability towards polar organic solvents and salt exposure, accepting mainly hydrophobic aromatic amine and carbonyl substrates. The 2.0 Å resolution crystal structure of VbTA T16F is reported, and together with computational calculations, revealed that this mutation is crucial for correct dimerisation and thus correct folding, leading to soluble protein expression.
Citation
Guidi, B., Planchestainer, M., Contente, M. L., Laurenzi, T., Eberini, I., Gourlay, L. J., …Molinari, F. (2018). Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form. https://doi.org/10.1038/s41598-018-34434-3
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 5, 2018 |
Online Publication Date | Nov 6, 2018 |
Publication Date | Dec 1, 2018 |
Deposit Date | Nov 13, 2018 |
Publicly Available Date | Nov 13, 2018 |
Journal | Scientific Reports |
Print ISSN | 2045-2322 |
Electronic ISSN | 2045-2322 |
Publisher | Nature Research |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 1 |
Article Number | 16441 |
DOI | https://doi.org/10.1038/s41598-018-34434-3 |
Public URL | https://nottingham-repository.worktribe.com/output/1246109 |
Publisher URL | https://www.nature.com/articles/s41598-018-34434-3 |
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
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