Reviving phage therapy for the treatment of cholera
Bhandare, Sudhakar; Colom, Joan; Baig, Abiyad; Ritchie, Jenny M.; Bokhari, Habib; Shah, Muhammad A.; Sarkar, B.L.; Su, Jingliang; Wren, Brendan; Barrow, Paul; Atterbury, Robert J.
Jenny M. Ritchie
Muhammad A. Shah
Robert J. Atterbury
Cholera remains a major risk in developing countries, particularly after natural or man-made disasters. Vibrio cholerae El Tor is the most important cause of these outbreaks, and is becoming increasingly resistant to antibiotics, so alternative therapies are urgently needed. In this study, a single bacteriophage, Phi_1, was used prophylactically and therapeutically to control cholera in an infant rabbit model. In both cases, phage-treated animals showed no clinical signs of disease, compared with 69% of untreated control animals. Bacterial counts in the intestines of phage-treated animals were reduced by up to 4 Log10 CFU/g. There was evidence of phage multiplication only in animals which received a V. cholerae challenge. No phage-resistant bacterial mutants were isolated from the animals, despite extensive searching. This is the first evidence that a single phage could be effective in the treatment of cholera, without detectable levels of resistance. Clinical trials in human patients should be considered.
|Journal Article Type||Article|
|Publication Date||Feb 15, 2019|
|Publisher||Oxford University Press|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Bhandare, S., Colom, J., Baig, A., Ritchie, J. M., Bokhari, H., Shah, M. A., …Atterbury, R. J. (2019). Reviving phage therapy for the treatment of cholera. Journal of Infectious Diseases, 219(5), 786–794. doi:10.1093/infdis/jiy563|
2018-09-17 JID-64864 R1 Repository AAM