Genomic and Phenotypic Analysis of Salmonella enterica Bacteriophages Identifies Two Novel Phage Species

Bhandare, Sudhakar; Lawal, Opeyemi U.; Colavecchio, Anna; Cadieux, Brigitte; Zahirovich-Jovich, Yella; Zhong, Zeyan; Tompkins, Elizabeth; Amitrano, Margot; Kukavica-Ibrulj, Irena; Boyle, Brian; Wang, Siyun; Levesque, Roger C.; Delaquis, Pascal; Danyluk, Michelle; Goodridge, Lawrence

Abstract

Bacteriophages (phages) are potential alternatives to chemical antimicrobials against pathogens of public health significance. Understanding the diversity and host specificity of phages is important for developing effective phage biocontrol approaches. Here, we assessed the host range, morphology, and genetic diversity of eight Salmonella enterica phages isolated from a wastewater treatment plant. The host range analysis revealed that six out of eight phages lysed more than 81% of the 43 Salmonella enterica isolates tested. The genomic sequences of all phages were determined. Whole-genome sequencing (WGS) data revealed that phage genome sizes ranged from 41 to 114 kb, with GC contents between 39.9 and 50.0%. Two of the phages SB13 and SB28 represent new species, Epseptimavirus SB13 and genera Macdonaldcampvirus, respectively, as designated by the International Committee for the Taxonomy of Viruses (ICTV) using genome-based taxonomic classification. One phage (SB18) belonged to the Myoviridae morphotype while the remaining phages belonged to the Siphoviridae morphotype. The gene content analyses showed that none of the phages possessed virulence, toxin, antibiotic resistance, type I–VI toxin–antitoxin modules, or lysogeny genes. Three (SB3, SB15, and SB18) out of the eight phages possessed tailspike proteins. Whole-genome-based phylogeny of the eight phages with their 113 homologs revealed three clusters A, B, and C and seven subclusters (A1, A2, A3, B1, B2, C1, and C2). While cluster C1 phages were predominantly isolated from animal sources, cluster B contained phages from both wastewater and animal sources. The broad host range of these phages highlights their potential use for controlling the presence of S. enterica in foods.

Citation

Bhandare, S., Lawal, O. U., Colavecchio, A., Cadieux, B., Zahirovich-Jovich, Y., Zhong, Z., Tompkins, E., Amitrano, M., Kukavica-Ibrulj, I., Boyle, B., Wang, S., Levesque, R. C., Delaquis, P., Danyluk, M., & Goodridge, L. (2024). Genomic and Phenotypic Analysis of Salmonella enterica Bacteriophages Identifies Two Novel Phage Species. Microorganisms, 12(4), Article 695. https://doi.org/10.3390/microorganisms12040695

• Journal Article Type: Article
• Acceptance Date: Mar 27, 2024
• Online Publication Date: Mar 29, 2024
• Publication Date: 2024-04
• Deposit Date: Mar 31, 2024
• Publicly Available Date: Apr 5, 2024
• Journal: Microorganisms
• Electronic ISSN: 2076-2607
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 4
• Article Number: 695
• DOI: https://doi.org/10.3390/microorganisms12040695
• Keywords: Salmonella; bacteriophage; diversity; host specificity; comparative phylogenomics; food safety
• Public URL: https://nottingham-repository.worktribe.com/output/33028254
• Publisher URL: https://www.mdpi.com/2076-2607/12/4/695