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Prognostic significance of tumour infiltrating B-Lymphocytes in breast ductal carcinoma in situ

Miligy, Islam; Mohan, Priya; Gaber, Ahmed; Aleskandarany, Mohammed A.; Nolan, Christopher C.; Diez?Rodriguez, Maria; Mukherjee, Abhik; Chapman, Caroline; Ellis, Ian O.; Green, Andrew R.; Rakha, Emad A.

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Islam Miligy

Priya Mohan

Ahmed Gaber

Mohammed A. Aleskandarany

Christopher C. Nolan

Maria Diez?Rodriguez

Caroline Chapman

Professor of Breast Cancer Pathology


Aims: Tumour?infiltrating lymphocytes (TILs) are an important component of the immune response to cancer and have a prognostic value in breast cancer. Although several studies have investigated the role of T lymphocytes in breast cancer, the role of B lymphocytes (TIL?Bs) in ductal carcinoma in situ (DCIS) remains uncertain. This study aimed to assess the role of TIL?Bs in DCIS.
Methods and results: Eighty DCIS cases (36 pure DCIS and 44 mixed with invasive cancer) were stained immunohistochemically for B lineage markers CD19, CD20 and the plasma cell marker CD138. TIL?Bs density and localization were assessed, including relation to the in?situ and invasive components. An association with clinicopathological data and patient outcome was performed. Pure DCIS showed a higher number of TIL?Bs and lymphoid aggregates than DCIS associated with invasion. In pure DCIS, a higher number of peri? and paratumoral TIL?Bs was associated significantly with large tumour size (P = 0.016), hormone receptor (ER/PR) negative (P = 0.008) and HER2+ status (P = 0.010). In tumours with mixed DCIS and invasive components, cases with high?density B lymphocytes, irrespective of their location or topographic distribution, were associated significantly with variables of poor prognosis, including larger size, high grade, lymphovascular invasion, lymph node metastases, ER/PR?negative and HER2+ status. Outcome analysis showed that pure DCIS associated with higher numbers of B lymphocytes had shorter recurrence?free interval (P = 0.04); however, the association was not significant with the CD138+ plasma cell count (P = 0.07).
Conclusion: Assessment of TIL?B cells based on location and topographic distribution can provide prognostic information. Validation in a larger cohort is warranted.


Miligy, I., Mohan, P., Gaber, A., Aleskandarany, M. A., Nolan, C. C., Diez‐Rodriguez, M., …Rakha, E. A. (2017). Prognostic significance of tumour infiltrating B-Lymphocytes in breast ductal carcinoma in situ. Histopathology, 71(2), 258-268.

Journal Article Type Article
Acceptance Date Mar 18, 2017
Online Publication Date Mar 22, 2017
Publication Date Aug 31, 2017
Deposit Date May 13, 2018
Publicly Available Date Nov 6, 2018
Print ISSN 0309-0167
Electronic ISSN 1365-2559
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 71
Issue 2
Pages 258-268
Public URL
Publisher URL
PMID 28326600


Final Revision Prognostic Significance Of Tumour Infiltrating B-Lymphocytes In Breast Ductal Carcinoma In Situ 9-3-2017 (905 Kb)

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