Mapping and validation of quantitative trait loci associated with concentrations of 16 elements in unmilled rice grain

Zhang, Min; Pinson, Shannon R.M.; Tarpley, Lee; Huang, Xin-Yuan; Lahner, Brett; Yakubova, Elena; Baxter, Ivan; Guerinot, Mary Lou; Salt, David E.

Abstract

Key Message: QTLs controlling the concentrations elements in rice grain were identified in two mapping populations. The QTLs were clustered such that most genomic regions were associated with more than one element.

In this study, quantitative trait loci (QTLs) affecting the concentrations of 16 elements in whole, unmilled rice (Oryza sativa L.) grain were identified. Two rice mapping populations, the 'Lemont' × 'TeQing' recombinant inbred lines (LT-RILs), and the TeQing-into-Lemont backcross introgression lines (TILs) were used. To increase opportunity to detect and characterize QTLs, the TILs were grown under two contrasting field conditions, flooded and irrigated-but-unflooded. Correlations between the individual elements and between each element with grain shape, plant height, and time of heading were also studied. Transgressive segregation was observed among the LT-RILs for all elements. The 134 QTLs identified as associated with the grain concentrations of individual elements were found clustered into 39 genomic regions, 34 of which were found associated with grain element concentration in more than one population and/or flooding treatment. More QTLs were found significant among flooded TILs (92) than among unflooded TILs (47) or among flooded LT-RILs (40). Twenty-seven of the 40 QTLs identified among the LT-RILs were associated with the same element among the TILs. At least one QTL per element was validated in two or more population/environments. Nearly all of the grain element loci were linked to QTLs affecting additional elements, supporting the concept of element networks within plants. Several of the grain element QTLs co-located with QTLs for grain shape, plant height, and days to heading; but did not always differ for grain elemental concentration as predicted by those traits alone. A number of interesting patterns were found, including a strong Mg-P-K complex. © 2013 The Author(s).

Citation

Zhang, M., Pinson, S. R., Tarpley, L., Huang, X., Lahner, B., Yakubova, E., …Salt, D. E. (2014). Mapping and validation of quantitative trait loci associated with concentrations of 16 elements in unmilled rice grain. TAG Theoretical and Applied Genetics, 127(1), 137-165. https://doi.org/10.1007/s00122-013-2207-5

• Journal Article Type: Article
• Acceptance Date: Oct 3, 2013
• Online Publication Date: Nov 15, 2013
• Publication Date: 2014-01
• Deposit Date: Sep 1, 2017
• Publicly Available Date: Feb 15, 2019
• Journal: Theoretical and Applied Genetics
• Print ISSN: 0040-5752
• Electronic ISSN: 1432-2242
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 127
• Issue: 1
• Pages: 137-165
• DOI: https://doi.org/10.1007/s00122-013-2207-5
• Public URL: https://nottingham-repository.worktribe.com/output/1117103
• Publisher URL: https://link.springer.com/article/10.1007%2Fs00122-013-2207-5
• PMID: 00033043