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Tocopherol: an intrinsic component of sunflower seed oil bodies

Fisk, Ian D.; White, Daniel A.; Carvalho, Andre; Gray, David A.

Authors

Daniel A. White

Andre Carvalho

DAVID GRAY david.gray@nottingham.ac.uk
Professor of Applied Lipid Science



Abstract

Oil bodies were removed from mature sunflower through wet grinding followed by filtration then centrifugation and recovered as the buoyant fraction. Washing this fraction with buffer (water‐washed oil bodies, WWOB) or 9 M urea (urea‐washed oil bodies, UWOB) resulted in the removal of extraneous proteins. SDS‐PAGE of the proteins still associated with the oil body fraction after washing indicated that this effect was particularly dramatic with urea washing. Thirty‐eight percent of the total seed tocopherol was recovered in WWOB after only one cycle of oil body recovery. The total phenolic content (TPC) of differentially washed sunflower seed oil bodies was used as a marker for the nonspecific association of phenolic compounds to oil bodies. This value decreased with increased removal of proteins from oil bodies, whereas the converse was true for tocopherol values, which increased from 214 mg total tocopherol kg−1 WWOB [dry wt basis (dwb)] to 392 mg total tocopherol kg−1 UWOB (dwb). The ratio of the four tocopherol isomers remained constant in the seed and oil body preparations (α:β:γ:δ approximately 94∶5∶0.5∶0.5). This work provides evidence that an intrinsic population of tocopherol molecules exists in the oil bodies of mature sunflower seeds.

Citation

Fisk, I. D., White, D. A., Carvalho, A., & Gray, D. A. (2006). Tocopherol: an intrinsic component of sunflower seed oil bodies. Journal of the American Oil Chemists' Society, 83(4), 341-344. https://doi.org/10.1007/s11746-006-1210-2

Journal Article Type Article
Acceptance Date Jan 1, 2006
Publication Date Apr 1, 2006
Deposit Date Sep 20, 2018
Journal Journal of the American Oil Chemists' Society
Print ISSN 0003-021X
Electronic ISSN 1558-9331
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 83
Issue 4
Pages 341-344
DOI https://doi.org/10.1007/s11746-006-1210-2
Keywords Oil body; Sunflower; Tocopherol
Public URL https://nottingham-repository.worktribe.com/output/1041848
Publisher URL https://onlinelibrary.wiley.com/doi/pdf/10.1007/s11746-006-1210-2