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Scanning electron microscopy of biomaterials

McKinlay, K.J.; Scotchford, C.A.; Grant, D.M.; Oliver, J.M.; King, John R.; Brown, Paul D.

Authors

K.J. McKinlay

C.A. Scotchford

D.M. Grant

J.M. Oliver

John R. King

Paul D. Brown



Contributors

Stephen McVitie
Editor

David McComb
Editor

Abstract

A comparison of conventional high vacuum scanning electron microscopy (HVSEM), environmental SEM (ESEM) and confocal laser scanning microscopy (CLSM) in the assessment of cell-material interactions is made. The processing of cells cultured for conventional HVSEM leads to the loss of morphological features that are retained when using ESEM. The use of ESEM in conjunction with CLSM of the labeled cytoskeleton gives an indication of changes to the cell morphology as a consequence of incubation time and substrate surface features.

Publication Date Jan 1, 2004
Peer Reviewed Peer Reviewed
Issue 179
Series Title Institute of Physics conference series
Book Title Electron Microscopy and Analysis 2003: Proceedings of the Institute of Physics Electron Microscopy and Analysis Group Conference, University of Oxford, 3-5 September 2003
ISBN 750309679
APA6 Citation McKinlay, K., Scotchford, C., Grant, D., Oliver, J., King, J. R., & Brown, P. D. (2004). Scanning electron microscopy of biomaterials. In S. McVitie, & D. McComb (Eds.), Electron Microscopy and Analysis 2003: Proceedings of the Institute of Physics Electron Microscopy and Analysis Group Conference, University of Oxford, 3-5 September 2003Institute of Physics Publishing
Keywords SEM
ESEM
CLSM
Biomaterials
Related Public URLs http://iopscience.iop.org/
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Inst._Phys._Conf._Ser._179_(2004)_pp_87-90.pdf (353 Kb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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