K.J. McKinlay
Scanning electron microscopy of biomaterials
McKinlay, K.J.; Scotchford, C.A.; Grant, D.M.; Oliver, J.M.; King, John R.; Brown, Paul D.
Authors
COLIN SCOTCHFORD COLIN.SCOTCHFORD@NOTTINGHAM.AC.UK
Associate Professor
D.M. Grant
J.M. Oliver
John R. King
Paul D. Brown
Contributors
Stephen McVitie
Editor
David McComb
Editor
Abstract
A comparison of conventional high vacuum scanning electron microscopy (HVSEM), environmental SEM (ESEM) and confocal laser scanning microscopy (CLSM) in the assessment of cell-material interactions is made. The processing of cells cultured for conventional HVSEM leads to the loss of morphological features that are retained when using ESEM. The use of ESEM in conjunction with CLSM of the labeled cytoskeleton gives an indication of changes to the cell morphology as a consequence of incubation time and substrate surface features.
Citation
McKinlay, K., Scotchford, C., Grant, D., Oliver, J., King, J. R., & Brown, P. D. (2004). Scanning electron microscopy of biomaterials. In S. McVitie, & D. McComb (Eds.), Electron Microscopy and Analysis 2003: Proceedings of the Institute of Physics Electron Microscopy and Analysis Group Conference, University of Oxford, 3-5 September 2003. Institute of Physics Publishing
Publication Date | Jan 1, 2004 |
---|---|
Deposit Date | Mar 25, 2011 |
Publicly Available Date | Mar 25, 2011 |
Peer Reviewed | Peer Reviewed |
Issue | 179 |
Series Title | Institute of Physics conference series |
Book Title | Electron Microscopy and Analysis 2003: Proceedings of the Institute of Physics Electron Microscopy and Analysis Group Conference, University of Oxford, 3-5 September 2003 |
ISBN | 750309679 |
Keywords | SEM ESEM CLSM Biomaterials |
Public URL | https://nottingham-repository.worktribe.com/output/1021674 |
Related Public URLs | http://iopscience.iop.org/ |
Files
Inst._Phys._Conf._Ser._179_(2004)_pp_87-90.pdf
(353 Kb)
PDF
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