5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary

Alioui, Anthony; Wheldon, Lee M.; Abakir, Abdulkadir; Ferjentsik, Zoltan; Johnson, Andrew D.; Ruzov, Alexey

Abstract

5-Methylcytosine (5-mC) is an epigenetic modification associated with gene repression. Recent studies demonstrated that 5-mC can be enzymatically oxidised into 5-hydroxymethylcytosine and further into 5-formylcytosine (5-fC) and 5-carboxylcytsine (5-caC). 5-caC has been found in embryonic stem cells and in mouse pre-implantation embryos but no detectable levels of this modification have been reported for somatic tissues to date. Whereas it has been suggested that 5-caC can serve as an intermediate in the process of active demethylation, the function of this form of modified cytosine remains obscure. Here we show that 5-caC is immunochemically detectable in somatic cells of axolotl ovary. We demonstrate that both 5-hmC and 5-caC are localized to the euchromatin in the nuclei of axolotl follicular cells with similar patterns of spatial distribution. Our results suggest that 5-carboxylcytosine may play a distinct functional role in certain biological contexts.

Citation

Alioui, A., Wheldon, L. M., Abakir, A., Ferjentsik, Z., Johnson, A. D., & Ruzov, A. (2012). 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary. Nucleus, 3(6), https://doi.org/10.4161/nucl.22799

• Journal Article Type: Article
• Publication Date: Nov 1, 2012
• Deposit Date: Mar 27, 2014
• Publicly Available Date: Mar 27, 2014
• Journal: Nucleus
• Print ISSN: 1949-1034
• Electronic ISSN: 1949-1042
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 6
• DOI: https://doi.org/10.4161/nucl.22799
• Public URL: https://nottingham-repository.worktribe.com/output/1006109
• Publisher URL: https://www.landesbioscience.com/journals/nucleus/article/22799/