Hiten D. Mistry firstname.lastname@example.org
Urine protein concentration estimation for biomarker discovery
Mistry, Hiten D.; Bramham, Kate; Weston, Andrew; Ward, Malcolm; Thompson, Andrew; Chappell, Lucy C.
Lucy C. Chappell
Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.
|Journal Article Type||Article|
|Publication Date||Oct 1, 2013|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Mistry, H. D., Bramham, K., Weston, A., Ward, M., Thompson, A., & Chappell, L. C. (2013). Urine protein concentration estimation for biomarker discovery. Pregnancy Hypertension, 3(4), doi:10.1016/j.preghy.2013.07.001|
|Keywords||Protein concentration assays; Proteomics; Urine|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0|
Tech note accepted.pdf
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
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