Urine protein concentration estimation for biomarker discovery

Mistry, Hiten D.; Bramham, Kate; Weston, Andrew; Ward, Malcolm; Thompson, Andrew; Chappell, Lucy C.

Abstract

Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.

Citation

Mistry, H. D., Bramham, K., Weston, A., Ward, M., Thompson, A., & Chappell, L. C. (2013). Urine protein concentration estimation for biomarker discovery. Pregnancy Hypertension, 3(4), https://doi.org/10.1016/j.preghy.2013.07.001

• Journal Article Type: Article
• Acceptance Date: Jul 25, 2013
• Online Publication Date: Aug 5, 2013
• Publication Date: Oct 1, 2013
• Deposit Date: Jul 21, 2017
• Publicly Available Date: Jul 21, 2017
• Journal: Pregnancy Hypertension
• Print ISSN: 2210-7789
• Electronic ISSN: 2210-7797
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 4
• DOI: https://doi.org/10.1016/j.preghy.2013.07.001
• Keywords: Protein concentration assays; Proteomics; Urine
• Public URL: https://nottingham-repository.worktribe.com/output/1001113
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S2210778913001980