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Urine protein concentration estimation for biomarker discovery

Mistry, Hiten D.; Bramham, Kate; Weston, Andrew; Ward, Malcolm; Thompson, Andrew; Chappell, Lucy C.

Authors

Hiten D. Mistry hiten.mistry@nottingham.ac.uk

Kate Bramham

Andrew Weston

Malcolm Ward

Andrew Thompson

Lucy C. Chappell



Abstract

Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.

Journal Article Type Article
Publication Date Oct 1, 2013
Journal Pregnancy Hypertension
Print ISSN 2210-7789
Electronic ISSN 2210-7797
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 3
Issue 4
APA6 Citation Mistry, H. D., Bramham, K., Weston, A., Ward, M., Thompson, A., & Chappell, L. C. (2013). Urine protein concentration estimation for biomarker discovery. Pregnancy Hypertension, 3(4), doi:10.1016/j.preghy.2013.07.001
DOI https://doi.org/10.1016/j.preghy.2013.07.001
Keywords Protein concentration assays; Proteomics; Urine
Publisher URL http://www.sciencedirect.com/science/article/pii/S2210778913001980
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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