@article { , title = {Urine protein concentration estimation for biomarker discovery}, abstract = {Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.}, doi = {10.1016/j.preghy.2013.07.001}, eissn = {2210-7797}, issn = {2210-7789}, issue = {4}, journal = {Pregnancy Hypertension}, publicationstatus = {Published}, publisher = {Elsevier}, url = {https://nottingham-repository.worktribe.com/output/1001113}, volume = {3}, keyword = {Protein concentration assays, Proteomics, Urine}, year = {2013}, author = {Mistry, Hiten D. and Bramham, Kate and Weston, Andrew and Ward, Malcolm and Thompson, Andrew and Chappell, Lucy C.} }