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Urine protein concentration estimation for biomarker discovery

Mistry, Hiten D.; Bramham, Kate; Weston, Andrew; Ward, Malcolm; Thompson, Andrew; Chappell, Lucy C.

Authors

Hiten D. Mistry hiten.mistry@nottingham.ac.uk

Kate Bramham

Andrew Weston

Malcolm Ward

Andrew Thompson

Lucy C. Chappell



Abstract

Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.

Citation

Mistry, H. D., Bramham, K., Weston, A., Ward, M., Thompson, A., & Chappell, L. C. (2013). Urine protein concentration estimation for biomarker discovery. Pregnancy Hypertension, 3(4), https://doi.org/10.1016/j.preghy.2013.07.001

Journal Article Type Article
Acceptance Date Jul 25, 2013
Online Publication Date Aug 5, 2013
Publication Date Oct 1, 2013
Deposit Date Jul 21, 2017
Publicly Available Date Jul 21, 2017
Journal Pregnancy Hypertension
Print ISSN 2210-7789
Electronic ISSN 2210-7797
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 3
Issue 4
DOI https://doi.org/10.1016/j.preghy.2013.07.001
Keywords Protein concentration assays; Proteomics; Urine
Public URL http://eprints.nottingham.ac.uk/id/eprint/44323
Publisher URL http://www.sciencedirect.com/science/article/pii/S2210778913001980
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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