Outputs (14)

Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis (2016)
Journal Article
Harding, S. E., Gillis, R. B., & Adams, G. G. (in press). Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis. Biophysical Reviews, 8(4), https://doi.org/10.1007/s12551-016-0232-8

Molecular weights (molar masses), molecular weight distributions, dissociation constants and other interaction parameters are fundamental characteristics of proteins, nucleic acids, polysaccharides and glycoconjugates in solution. Sedimentation equil... Read More about Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis.

Foreword to ‘Quantitative and analytical relations in biochemistry’—a special issue in honour of Donald J. Winzor’s 80th birthday (2016)
Journal Article
Hall, D., & Harding, S. E. (2016). Foreword to ‘Quantitative and analytical relations in biochemistry’—a special issue in honour of Donald J. Winzor’s 80th birthday. Biophysical Reviews, 8(4), 269-277. https://doi.org/10.1007/s12551-016-0227-5

The purpose of this special issue is to honour Professor Donald J. Winzor’s long career as a researcher and scientific mentor, and to celebrate the milestone of his 80th birthday. Throughout his career, Don has been renowned for his development of cl... Read More about Foreword to ‘Quantitative and analytical relations in biochemistry’—a special issue in honour of Donald J. Winzor’s 80th birthday.

Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein (2016)
Journal Article
Abdelhameed, A. S., Morris, G. A., Almutairi, F., Adams, G. G., Duvivier, P., Conrath, K., & Harding, S. E. (in press). Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein. Scientific Reports, 6(1), 1-11. https://doi.org/10.1038/srep35588

The structural integrity of meningococcal native, micro-fluidized and activated capsular polysaccharides and their glycoconjugates – in the form most relevant to their potential use as vaccines (dilute solution) - have been investigated with respect... Read More about Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein.

Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions (2016)
Journal Article
Sarangapani, P. S., Weaver, J., Parupudi, A., Besong, T. M., Adams, G. G., Harding, S. E., Manikwar, P., Castellanos, M. M., Bishop, S. M., & Pathak, J. A. (2016). Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions. Journal of Pharmaceutical Sciences, 105(12), https://doi.org/10.1016/j.xphs.2016.08.020

The role of antibody structure (conformation) in solution rheology is probed. It is demonstrated here that pH-dependent changes in the tertiary structure of 2 mAb solutions lead to viscoelasticity and not merely a shear viscosity (η) increase. Steady... Read More about Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions.

Genetic improvement of tomato by targeted control of fruit softening (2016)
Journal Article
Uluisik, S., Chapman, N. H., Smith, R., Poole, M., Adams, G., Gillis, R. B., Besong, T. D., Sheldon, J., Stiegelmeyer, S., Perez, L., Samsulrizi, N., Wang, D., Fisk, I. D., Yang, N., Baxter, C., Rickett, D., Fray, R., Blanco-Ulate, B., Powell, A. L., Harding, S. E., …Seymour, G. B. (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34, 950-952. https://doi.org/10.1038/nbt.3602

Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' t... Read More about Genetic improvement of tomato by targeted control of fruit softening.

Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans (2016)
Journal Article
Gillis, R. B., Adams, G. G., Besong, D. T., Machová, E., Ebringerová, A., Rowe, A. J., Harding, S. E., & Patel, T. R. (2017). Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans. European Biophysics Journal, 46(3), 235-245. https://doi.org/10.1007/s00249-016-1159-5

Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently develope... Read More about Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans.

A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic (2016)
Journal Article
Gillis, R. B., Adams, G. G., Alzahrani, Q., Harding, S. E., & Allen Bush, C. (2016). A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic. Biopolymers, 105(9), 618-625. https://doi.org/10.1002/bip.22823

Under investigation are the structural properties of gum arabic, an industrially important biopolymer for use as a stabilizer or in drug delivery, using Analytical Ultracentrifugation—a well-established, matrix-free probe for macromolecular size and... Read More about A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic.

Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions (2016)
Journal Article
Hussain, R., Harding, S. E., Hughes, C. S., Ma, P., Patching, S. G., Edara, S., Siligardi, G., Henderson, P. J., & Phillips-Jones, M. K. (2016). Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions. Biochemical Society Transactions, 44(3), 810-823. https://doi.org/10.1042/BST20160023

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction famil... Read More about Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions.

The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health (2016)
Journal Article
Horniblow, R. D., Latunde-Dada, G. O., Harding, S. E., Schneider, M., Almutairi, F. M., Sahni, M., Bhatti, A., Ludwig, C., Norton, I. T., Iqbal, T. H., & Tselepis, C. (2016). The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health. Molecular Nutrition and Food Research, 60(9), 2098-2108. https://doi.org/10.1002/mnfr.201500882

Scope

Iron is an essential nutrient. However, in animal models, excess unabsorbed dietary iron residing within the colonic lumen has been shown to exacerbate inflammatory bowel disease and intestinal cancer. Therefore, the aims of this study were... Read More about The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health.

Molecular weight analysis of starches: which technique? (2016)
Journal Article
Harding, S. E., Adams, G. G., & Gillis, R. B. (2016). Molecular weight analysis of starches: which technique?. Starch/Stärke, 68(9-10), https://doi.org/10.1002/star.201600042

A number of methods are available to researchers for estimating molecular weights (molar masses) and molecular weight distributions of starches − or those in solution, using an appropriate solvent/solubilisation protocol. We outline the methods avail... Read More about Molecular weight analysis of starches: which technique?.