Outputs (24)

An enzymatically controlled mucoadhesive for enhancing flavour during food oral processing (2019)
Journal Article
Dinu, V., Gadon, A., Hurst, K., Lim, M., Ayed, C., Gillis, R. B., Adams, G. G., Harding, S. E., & Fisk, I. D. (2019). An enzymatically controlled mucoadhesive for enhancing flavour during food oral processing. npj Science of Food, 3(1), Article 11. https://doi.org/10.1038/s41538-019-0043-y

While a good mucoadhesive biopolymer must adhere to a mucus membrane, it must also have a good unloading ability. Here, we demonstrate that the biopolymer pullulan is partially digested by human salivary α-amylase, thus acting as a controlled release... Read More about An enzymatically controlled mucoadhesive for enhancing flavour during food oral processing.

Use of the extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans (2018)
Journal Article
Channell, G. A., Adams, G. G., Lu, Y., Gillis, R. B., Dinu, V., Grundy, M. M.-L., Bajka, B., Butterworth, P. J., Ellis, P. R., Mackie, A., Ballance, S., & Harding, S. E. (2018). Use of the extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans. Scientific Reports, 8(1), Article 11809. https://doi.org/10.1038/s41598-018-29997-0

Beta 1–3, 1–4 glucans (“beta-glucans”) are one of the key components of the cell wall of cereals, complementing the main structural component cellulose. Beta-glucans are also an important source of soluble fibre in foods containing oats with claims o... Read More about Use of the extended Fujita method for representing the molecular weight and molecular weight distributions of native and processed oat beta-glucans.

The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science (2018)
Journal Article
Harding, S. E., Wakefield, J., Gillis, R., Adams, G., & McQueen, C. (2018). The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science. European Biophysics Journal, 47(7), 697–707. https://doi.org/10.1007/s00249-018-1290-6

The analytical ultracentrifuge (AUC) invented by T. Svedberg has now become an extremely versatile and diverse tool in Biochemistry and Polymer Science for the characterisation of the sizes, shapes and interactions of particles ranging in size from a... Read More about The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science.

Characterisation of insulin analogues therapeutically available to patients (2018)
Journal Article
Adams, G. G., Meal, A., Morgan, P. S., Alzahrani, Q. E., Zobel, H., Lithgo, R., Kok, M. S., Besong, D. T., Jiwani, S. I., Ballance, S., Harding, S. E., Gillis, R. B., & Chayen, N. (2018). Characterisation of insulin analogues therapeutically available to patients. PLoS ONE, 13(3), Article e0195010. https://doi.org/10.1371/journal.pone.0195010

The structure and function of clinical dosage insulin and its analogues were assessed. This included ‘native insulins’ (human recombinant, bovine, porcine), ‘fast-acting analogues’ (aspart, glulisine, lispro) and ‘slow-acting analogues’ (glargine, de... Read More about Characterisation of insulin analogues therapeutically available to patients.

Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation (2018)
Journal Article
Wakefield, J. M., Gillis, R. B., Adams, G. G., McQueen, C., & Harding, S. E. (2018). Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation. European Biophysics Journal, 47(7), 769-775. https://doi.org/10.1007/s00249-018-1290-6

The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1), for potential use as a consolidant for decayed archaeological wood, has been examined by sedimentation velocity and sedimen... Read More about Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation.

Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration (2017)
Journal Article
Phillips-Jones, M. K., Lithgo, R., Dinu, V., Gillis, R. B., Harding, J. E., Adams, G. G., & Harding, S. E. (2017). Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration. Scientific Reports, 7(1), 1-10. https://doi.org/10.1038/s41598-017-12620-z

The reversibility and strength of the previously established dimerization of the important glycopeptide antibiotic vancomycin in four different aqueous solvents (including a medically-used formulation) have been studied using short-column sedimentati... Read More about Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration.

Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin (2017)
Journal Article
Phillips-Jones, M. K., Channell, G. A., Kelsall, C. J., Hughes, C. S., Ashcroft, A. E., Patching, S. G., Dinu, V., Gillis, R. B., Adams, G. G., & Harding, S. E. (2017). Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin. Scientific Reports, 7(1), 1-12. https://doi.org/10.1038/srep46180

VanA-type resistance to glycopeptide antibiotics in clinical enterococci is regulated by the VanSARA two-component signal transduction system. The nature of the molecular ligand that is recognised by the VanSA sensory component has not hitherto been... Read More about Hydrodynamics of the VanA-type VanS histidine kinase: an extended solution conformation and first evidence for interactions with vancomycin.

Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis (2016)
Journal Article
Harding, S. E., Gillis, R. B., & Adams, G. G. (in press). Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis. Biophysical Reviews, 8(4), https://doi.org/10.1007/s12551-016-0232-8

Molecular weights (molar masses), molecular weight distributions, dissociation constants and other interaction parameters are fundamental characteristics of proteins, nucleic acids, polysaccharides and glycoconjugates in solution. Sedimentation equil... Read More about Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis.

Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein (2016)
Journal Article
Abdelhameed, A. S., Morris, G. A., Almutairi, F., Adams, G. G., Duvivier, P., Conrath, K., & Harding, S. E. (in press). Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein. Scientific Reports, 6(1), 1-11. https://doi.org/10.1038/srep35588

The structural integrity of meningococcal native, micro-fluidized and activated capsular polysaccharides and their glycoconjugates – in the form most relevant to their potential use as vaccines (dilute solution) - have been investigated with respect... Read More about Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein.

Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions (2016)
Journal Article
Sarangapani, P. S., Weaver, J., Parupudi, A., Besong, T. M., Adams, G. G., Harding, S. E., Manikwar, P., Castellanos, M. M., Bishop, S. M., & Pathak, J. A. (2016). Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions. Journal of Pharmaceutical Sciences, 105(12), https://doi.org/10.1016/j.xphs.2016.08.020

The role of antibody structure (conformation) in solution rheology is probed. It is demonstrated here that pH-dependent changes in the tertiary structure of 2 mAb solutions lead to viscoelasticity and not merely a shear viscosity (η) increase. Steady... Read More about Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions.