Outputs (20)

Crystal structure of coagulation factor XII N-terminal domains 1–5 (2025)
Journal Article
Saleem, M., Li, C., Kaira, B. G., Brown, A. K., Pathak, M., Najmudin, S., Cowieson, N., Dreveny, I., Wilson, C., Shamanaev, A., Gailani, D., Smith, S. A., Morrissey, J. H., Philippou, H., & Emsley, J. (2025). Crystal structure of coagulation factor XII N-terminal domains 1–5. Acta Crystallographica. Section D, Structural Biology, 81(7), 380-393. https://doi.org/10.1107/s2059798325005297

Factor XIIa (FXIIa) is generated from its zymogen factor XII (FXII) by contact with polyanions such as inorganic polyphosphates. FXIIa cleaves the substrates prekallikrein and factor XI, triggering inflammatory cascades and plasma coagulation. From t... Read More about Crystal structure of coagulation factor XII N-terminal domains 1–5.

A versatile fluorescence polarization-based deubiquitination assay using an isopeptide bond substrate mimetic (IsoMim) (2025)
Journal Article
Zhang, J., Allen, J., Ward, S. J., Dekker, L. V., & Dreveny, I. (2025). A versatile fluorescence polarization-based deubiquitination assay using an isopeptide bond substrate mimetic (IsoMim). Journal of Biological Chemistry, 301(7), Article 110342. https://doi.org/10.1016/j.jbc.2025.110342

Deubiquitinases (DUBs) play a critical role in the regulation of various cellular processes, such as protein homeostasis and signaling, rendering them attractive drug targets. However, the generation of reagents for measuring DUB activity typically i... Read More about A versatile fluorescence polarization-based deubiquitination assay using an isopeptide bond substrate mimetic (IsoMim).

Ubiquitin-specific protease 11 structure in complex with an engineered substrate mimetic reveals a molecular feature for deubiquitination selectivity (2023)
Journal Article
Maurer, S. K., Mayer, M. P., Ward, S. J., Boudjema, S., Halawa, M., Zhang, J., Caulton, S. G., Emsley, J., & Dreveny, I. (2023). Ubiquitin-specific protease 11 structure in complex with an engineered substrate mimetic reveals a molecular feature for deubiquitination selectivity. Journal of Biological Chemistry, 299(11), Article 105300. https://doi.org/10.1016/j.jbc.2023.105300

Ubiquitin-specific proteases (USPs) are crucial for controlling cellular proteostasis and signaling pathways but how deubiquitination is selective remains poorly understood, in particular between paralogues. Here, we developed a fusion tag method by... Read More about Ubiquitin-specific protease 11 structure in complex with an engineered substrate mimetic reveals a molecular feature for deubiquitination selectivity.

Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis (2023)
Journal Article
Ducker, C., French, S., Pathak, M., Taylor, H., Sainter, A., Askem, W., Dreveny, I., Goulart Santana, A. E., Pickett, J. A., & Oldham, N. J. (2023). Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis. Insect Biochemistry and Molecular Biology, 161, Article 104001. https://doi.org/10.1016/j.ibmb.2023.104001

Leishmaniasis is a debilitating and often fatal neglected tropical disease. Males from sub-populations of the Leishmania-harbouring sandfly, Lutzomyia longipalpis, produce the diterpene sex and aggregation pheromone, sobralene, for which geranylgeran... Read More about Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis.

Structures of factor XI and prekallikrein bound to domain 6 of high–molecular weight kininogen reveal alternate domain 6 conformations and exosites (2023)
Journal Article
Li, C., Barroeta, A. B., Wong, S. S., Kim, H. J., Pathak, M., Dreveny, I., Meijers, J. C., & Emsley, J. (2023). Structures of factor XI and prekallikrein bound to domain 6 of high–molecular weight kininogen reveal alternate domain 6 conformations and exosites. Journal of Thrombosis and Haemostasis, https://doi.org/10.1016/j.jtha.2023.03.042

Background: High–molecular weight kininogen (HK) circulates in plasma as a complex with zymogen prekallikrein (PK). HK is both a substrate and a cofactor for activated plasma kallikrein, and the principal exosite interactions occur between PK N-termi... Read More about Structures of factor XI and prekallikrein bound to domain 6 of high–molecular weight kininogen reveal alternate domain 6 conformations and exosites.

Protein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase (2023)
Journal Article
Hicks, K. G., Cluntun, A. A., Schubert, H. L., Hackett, S. R., Berg, J. A., Leonard, P. G., Ajalla Aleixo, M. A., Zhou, Y., Bott, A. J., Salvatore, S. R., Chang, F., Blevins, A., Barta, P., Tilley, S., Leifer, A., Guzman, A., Arok, A., Fogarty, S., Winter, J. M., Ahn, H. C., …Rutter, J. (2023). Protein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase. Science, 379(6636), 996-1003. https://doi.org/10.1126/science.abm3452

Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry int... Read More about Protein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase.

Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia (2022)
Journal Article
Jin, Q., Gutierrez Diaz, B., Pieters, T., Zhou, Y., Narang, S., Fijalkwoski, I., Borin, C., Van Laere, J., Payton, M., Cho, B.-K., Han, C., Sun, L., Serafin, V., Yacu, G., Von Loocke, W., Basso, G., Veltri, G., Dreveny, I., Ben-Sahra, I., Goo, Y. A., …Ntziachristos, P. (2022). Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia. Science Advances, 8(49), https://doi.org/10.1126/sciadv.abq8437

Dysregulation of kinase signaling pathways favors tumor cell survival and therapy resistance in cancer. Here, we reveal a posttranslational regulation of kinase signaling and nuclear receptor activity via deubiquitination in T cell acute lymphoblasti... Read More about Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia.

Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases (2022)
Book Chapter
Spiliotopoulos, A., Maurer, S. K., Tsoumpeli, M. T., Bonfante, J. A. F., Owen, J. P., Gough, K. C., & Dreveny, I. (2023). Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases. In J. Maupin-Furlow, & M. J. Edelmann (Eds.), Deubiquitinases: Methods and Protocols (189-218). Humana Press. https://doi.org/10.1007/978-1-0716-2803-4_12

Phage display (PD) is a powerful method and has been extensively used to generate monoclonal antibodies and identify epitopes, mimotopes, and protein interactions. More recently, the combination of next-generation sequencing (NGS) with PD (NGPD) has... Read More about Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases.

Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair (2021)
Journal Article
Sanchez-Bailon, M. P., Choi, S. Y., Dufficy, E. R., Sharma, K., McNee, G. S., Gunnell, E., Chiang, K., Sahay, D., Maslen, S., Stewart, G. S., Skehel, J. M., Dreveny, I., & Davies, C. C. (2021). Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair. Nature Communications, 12(1), Article 6313. https://doi.org/10.1038/s41467-021-26413-6

Cross-talk between distinct protein post-translational modifications is critical for an effective DNA damage response. Arginine methylation plays an important role in maintaining genome stability, but how this modification integrates with other enzym... Read More about Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair.

Factor XII and kininogen asymmetric assembly with gC1qR/C1QBP/P32 is governed by allostery (2020)
Journal Article
Kaira, B. G., Slater, A., McCrae, K. R., Dreveny, I., Sumya, U.-M., Mutch, N. J., Searle, M., & Emsley, J. (2020). Factor XII and kininogen asymmetric assembly with gC1qR/C1QBP/P32 is governed by allostery. Blood, 136(14), 1685–1697. https://doi.org/10.1182/blood.2020004818

The contact system is composed of Factor XII (FXII), prekallikrein (PK) and co-factor kininogen (HK). The globular C1q receptor (gC1qR) has been shown to interact with FXII and HK. We reveal the FXII fibronectin type II domain (FnII) binds gC1qR in a... Read More about Factor XII and kininogen asymmetric assembly with gC1qR/C1QBP/P32 is governed by allostery.